Suemaru S, Hashimoto K, Ogasa T, Takao T, Ota Z, Hirakawa M, Kawata M
Department of Internal Medicine, Okayama University Medical School, Japan.
Acta Med Okayama. 1990 Oct;44(5):233-41. doi: 10.18926/AMO/30451.
The effects of salt loading and adrenalectomy on arginine vasopressin (AVP) mRNA levels in the paraventricular nucleus (PVN) and the supraoptic nucleus (SON) of the hypothalamus were studied by semiquantitative in situ hybridization histochemistry, using a synthetic oligonucleotide probe and a computer-assisted image analysis system. Salt loading (2% NaCl) for 7 days produced marked increases in AVP mRNA levels in the magnocellular neurons of the PVN, SON, and accessory nuclei. Adrenalectomy caused an increase in AVP mRNA expression in the magnocellular part of the PVN and the expansion of hybridization signals into its medial parvocellular region, where the cell bodies of corticotropin-releasing hormone (CRH) neurons are located. No apparent alteration of AVP mRNA levels was observed in the SON following adrenalectomy. These results indicate that hyperosmotic stimulation and the loss of circulating glucocorticoids had differential effects on AVP gene expression in the PVN and SON, and that the magnocellular PVN and SON neurons responded in different manners to the loss of feedback signals.
采用合成寡核苷酸探针和计算机辅助图像分析系统，通过半定量原位杂交组织化学技术，研究了盐负荷和肾上腺切除对下丘脑室旁核（PVN）和视上核（SON）中精氨酸加压素（AVP）mRNA水平的影响。给予7天的盐负荷（2% NaCl）可使PVN、SON和副核的大细胞神经元中AVP mRNA水平显著升高。肾上腺切除导致PVN大细胞部分的AVP mRNA表达增加，且杂交信号扩展至其内侧小细胞区域，该区域是促肾上腺皮质激素释放激素（CRH）神经元的胞体所在位置。肾上腺切除后，SON中AVP mRNA水平未观察到明显变化。这些结果表明，高渗刺激和循环糖皮质激素的丧失对PVN和SON中AVP基因表达具有不同的影响，且PVN和SON的大细胞神经元对反馈信号丧失的反应方式不同。
