比较通透离子摄取法和类胡萝卜素谱带位移法作为测定球形红假单胞菌Ga型菌色素小体膜电位的方法
Comparison of permeant ion uptake and carotenoid band shift as methods for determining the membrane potential in chromatophores from Rhodopseudomonas sphaeroides Ga.
作者信息
Ferguson S J, Jones O T, Kell D B, Sorgato M C
出版信息
Biochem J. 1979 Apr 15;180(1):75-85. doi: 10.1042/bj1800075.
Abstract
- A comparison was made of two methods for estimating the membrane potential in chromatophores from Rhodopseudomonas sphaeroides Ga. Illuminated chromatophores generated a potential that is apparently much larger when estimated on the basis of the red-band shift of carotenoids rather than from the extent of uptake of the permeant SCN- ion. 2. In contrast, when the chromatophores were oxidizing NADH or succinate the uptake of SCN- indicated a larger membrane potential than was estimated from the carotenoid band shift. 3. The extent of SCN- uptake and the carotenoid-band shift respond differently to changes in the ionic composition of the reaction medium. 4. The effects of antimycin on the carotenoid band shift and SCN- uptake are reported. 5. It is concluded that the carotenoid band shift and the uptake of SCN- are responding to different aspects of the energized state.
摘要
- 对两种估算球形红假单胞菌（Rhodopseudomonas sphaeroides Ga）载色体膜电位的方法进行了比较。光照下的载色体产生的电位，基于类胡萝卜素的红移估算时明显比基于渗透性硫氰酸根离子（SCN-）的摄取程度估算时要大得多。2. 相反，当载色体氧化还原型辅酶Ⅰ（NADH）或琥珀酸时，硫氰酸根离子的摄取表明膜电位比从类胡萝卜素谱带红移估算出的膜电位更大。3. 硫氰酸根离子的摄取程度和类胡萝卜素谱带红移对反应介质离子组成的变化反应不同。4. 报道了抗霉素对类胡萝卜素谱带红移和硫氰酸根离子摄取的影响。5. 得出的结论是，类胡萝卜素谱带红移和硫氰酸根离子的摄取对激发态的不同方面作出反应。
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本文引用的文献
1
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J Biol Chem. 1951 Nov;193(1):265-75.
2
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J Gen Microbiol. 1960 Jun;22:778-85. doi: 10.1099/00221287-22-3-778.
3
STUDIES ON A MUTANT OF RHODOPSEUDOMONAS SPHEROIDES UNABLE TO GROW PHOTOSYNTHETICALLY.球形红假单胞菌一个无法进行光合生长的突变体的研究
Biochim Biophys Acta. 1964 Jul 29;88:61-73. doi: 10.1016/0926-6577(64)90154-8.
4
The high energy state in chromatophores from Rhodopseudomonas spheroides.球形红假单胞菌色素细胞中的高能态。
FEBS Lett. 1969 Aug;4(3):185-189. doi: 10.1016/0014-5793(69)80230-9.
5
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6
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7
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8
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10
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