Iowa State University, Department of Entomology, Ames, IA 50011, USA.
J Econ Entomol. 2012 Apr;105(2):625-31. doi: 10.1603/ec11096.
The western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a major pest of corn (Zea mays L.) in North America and has evolved resistance to crop rotation by ovipositing in alternate crops such as soybeans [Glycine max (L.) Merr.]. Through experiments with plants grown in the greenhouse and the field, we tested whether soybeans with resistance to the soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), affected survival, fecundity, and consumption of soybean for D. v. virgifera. Soybean varieties tested included those types resistant to A. glycines (Rag1 and rag1/rag3) and a susceptible near isoline of the Rag1 variety. Females were provided with a diet of corn tissue for 4 d after which they were fed a diet of tissue from one of three soybean varieties for 4 d, starved for 4 d, or fed corn tissue. When fed greenhouse grown plants, strains differed significantly in survival and consumption, but consumption did not differ by variety of soybean. Diet treatment only affected fecundity; individuals fed corn continuously had greater fecundity than those individuals fed soybeans. In the experiment with plants grown in the field, leaf consumption differed among strains and individuals fed corn continuously had greater fecundity than the other treatments. Soybean varieties with Rag1 and rag1/rag3 resistance to A. glycines did not appear to affect the fitness of D. v. virgifera. Thus, planting of these A. glycines-resistant soybean varieties should not directly affect the spread of rotation-resistant D. v. virgifera.
西方玉米根萤叶甲，Diabrotica virgifera virgifera LeConte（鞘翅目：叶甲科），是北美洲玉米的主要害虫，通过在大豆[ Glycine max（L.）Merr.]等替代作物中产卵，已经对轮作产生了抗性。通过温室和田间植物实验，我们测试了对大豆蚜，Aphis glycines Matsumura（半翅目：蚜科）具有抗性的大豆是否会影响西方玉米根萤叶甲的生存、繁殖和对大豆的消耗。测试的大豆品种包括对 A. glycines 具有抗性的类型（Rag1 和 rag1/rag3）和 Rag1 品种的易感近等基因系。雌性在接受玉米组织 4 天后，被喂食三种大豆品种之一的组织 4 天，饥饿 4 天，或喂食玉米组织。当喂食温室种植的植物时，不同品系的存活率和消耗量有显著差异，但大豆品种不同并不影响消耗量。饮食处理仅影响繁殖力；连续喂食玉米的个体比喂食大豆的个体繁殖力更强。在田间种植植物的实验中，不同菌株之间的叶片消耗量存在差异，连续喂食玉米的个体比其他处理的个体繁殖力更强。对 A. glycines 具有 Rag1 和 rag1/rag3 抗性的大豆品种似乎不会影响西方玉米根萤叶甲的适应性。因此，种植这些抗 A. glycines 的大豆品种不应直接影响轮作抗性西方玉米根萤叶甲的传播。
