Tani Akiko, Tada Yasuhiro, Takezawa Toshiaki, Imaizumi Mitsuyoshi, Nomoto Yukio, Nakamura Tatsuo, Omori Koichi
Department of Otolaryngology, Fukushima Medical University, Fukushima, Japan.
Ann Otol Rhinol Laryngol. 2012 Apr;121(4):261-8. doi: 10.1177/000348941212100412.
Our group has had good results in tracheal mucosal regeneration using a collagen vitrigel-sponge scaffold in an animal model. In this study, the effectiveness of this scaffold with the application of basic fibroblast growth factor (b-FGF) was investigated.
A collagen vitrigel-sponge scaffold was fabricated with simultaneous addition of b-FGF. Three types of collagen vitrigel-sponge scaffolds were made: no b-FGF, 10 ng of b-FGF, and 100 ng of b-FGF. At 3, 5, 7, and 14 days after implantation in rats, the tracheas were removed and histologically evaluated. The regeneration of mucosal epithelium and the subepithelial layer was evaluated.
Mucosal epithelium, including pseudostratified epithelium and ciliated cells, regenerated earlier in the scaffolds when b-FGF was applied than when b-FGF was not applied. Regeneration of the subepithelial layer, infiltration of inflammatory cells and fibroblasts, and angiogenesis were promoted earlier in the scaffolds with b-FGF application.
Our technique for tracheal reconstruction using collagen vitrigel-sponge scaffolds with b-FGF application affords a feasible approach for accelerating the regeneration of the intraluminal surface and subepithelial layer of tracheal tissue.
我们的研究小组在动物模型中使用胶原玻璃体海绵支架进行气管黏膜再生取得了良好的效果。在本研究中，研究了该支架联合应用碱性成纤维细胞生长因子（b-FGF）的有效性。
制备同时添加b-FGF的胶原玻璃体海绵支架。制作三种类型的胶原玻璃体海绵支架：不添加b-FGF、添加10 ng b-FGF和添加100 ng b-FGF。在大鼠植入后3、5、7和14天，取出气管并进行组织学评估。评估黏膜上皮和上皮下层的再生情况。
与未应用b-FGF时相比，应用b-FGF时支架内黏膜上皮（包括假复层上皮和纤毛细胞）再生更早。应用b-FGF的支架中，上皮下层的再生、炎性细胞和成纤维细胞的浸润以及血管生成更早得到促进。
我们应用b-FGF的胶原玻璃体海绵支架进行气管重建的技术为加速气管组织腔内表面和上皮下层的再生提供了一种可行的方法。
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