钛合金表面氧化物调节吸附的纤连蛋白的构象，以增强其与成骨细胞中α(5)β(1)整合素的结合。
Titanium alloy surface oxide modulates the conformation of adsorbed fibronectin to enhance its binding to α(5) β(1) integrins in osteoblasts.
作者信息
Rapuano Bruce E, Lee Jani J E, MacDonald Daniel E
机构信息
Hospital for Special Surgery affiliated with the Weill Medical College of Cornell University, New York, NY 10021, USA.
出版信息
Eur J Oral Sci. 2012 Jun;120(3):185-94. doi: 10.1111/j.1600-0722.2012.954.x. Epub 2012 May 8.
Abstract
Our laboratory has previously demonstrated that heat (600°C) or radiofrequency plasma glow discharge (RFGD) pretreatment of a titanium alloy (Ti6Al4V) increased the net negative charge of the alloy's surface oxide and the attachment of osteoblastic cells to adsorbed fibronectin. The purpose of the current study was to investigate the biological mechanism by which these surface pretreatments enhance the capacity of fibronectin to stimulate osteoblastic cell attachment. Each pretreatment was found to increase the binding (measured by ELISA) of a monoclonal anti-fibronectin Ig to the central integrin-binding domain of adsorbed fibronectin, and to increase the antibody's inhibition of osteogenic cell attachment (measured by hexosaminidase assay). Pretreatments also increased the binding (measured by ELISA) of anti-integrin IgG's to the α(5) and β(1) integrin subunits that became attached to fibronectin during cell incubation. These findings suggest that negatively charged surface oxides of Ti6Al4V cause conformational changes in fibronectin that increase the availability of its integrin-binding domain to α(5) β(1) integrins.
摘要
我们实验室之前已经证明，对钛合金（Ti6Al4V）进行加热（600°C）或射频等离子体辉光放电（RFGD）预处理，会增加合金表面氧化物的净负电荷以及成骨细胞对吸附的纤连蛋白的附着。本研究的目的是探究这些表面预处理增强纤连蛋白刺激成骨细胞附着能力的生物学机制。发现每种预处理均增加了单克隆抗纤连蛋白Ig与吸附的纤连蛋白的中央整合素结合结构域的结合（通过ELISA测量），并增加了抗体对成骨细胞附着的抑制作用（通过己糖胺酶测定法测量）。预处理还增加了抗整合素IgG与在细胞孵育期间附着于纤连蛋白的α(5)和β(1)整合素亚基的结合（通过ELISA测量）。这些发现表明，Ti6Al4V带负电荷的表面氧化物会导致纤连蛋白发生构象变化，从而增加其整合素结合结构域对α(5)β(1)整合素的可用性。
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