Sardesai N Y, Stagg S M, Vanloock M S, Harvey S C, Schimmel P
a The Skaggs Institute for Chemical Biology, The Scripps Research Institute , 10550 North Torrey Pines Road , La Jolla , CA , 92037.
J Biomol Struct Dyn. 2000;17 Suppl 1:29-37. doi: 10.1080/07391102.2000.10506601.
Abstract Some evidence and considerations suggest that RNA minihelices based on the acceptor-TΨC stem-loop of tRNAs are the historical, more ancient part of the tRNA structure. These minihelices are substrates for aminoacylation by tRNA synthetases. In the transition from the RNA world to the theatre of proteins, aminoacyl minihelices may have had a role in early systems of peptide synthesis. Such systems would require bringing together aminoacyl groups into close proximity in order for peptide bonds to form. Here we report the design of RNA scaffolds based on pieces of the structure of the P4-P6 domain of the Tetrahymena ribozyme. RNA minihelices were incorporated into these scaffolds and the resulting RNAs could be enzymatically aminoacylated. The RNA scaffolds containing the minihelix-like pieces associated spontaneously to create the presumptive P4-P6 structure and thereby bring together the substrates for aminoacylation. Thus, peptide synthesis with associating RNA scaffolds that contain minihelix-like motifs appears plausible.
摘要 一些证据和思考表明，基于tRNA受体-TΨC茎环的RNA小螺旋是tRNA结构中历史更悠久、更古老的部分。这些小螺旋是tRNA合成酶进行氨酰化的底物。在从RNA世界向蛋白质世界的转变过程中，氨酰化小螺旋可能在早期肽合成系统中发挥了作用。这样的系统需要将氨酰基紧密聚集在一起才能形成肽键。在此，我们报道了基于四膜虫核酶P4-P6结构域片段设计的RNA支架。将RNA小螺旋整合到这些支架中，所得RNA可被酶促氨酰化。含有类小螺旋片段的RNA支架会自发结合以形成假定的P4-P6结构，从而将氨酰化底物聚集在一起。因此，利用含有类小螺旋基序的结合型RNA支架进行肽合成似乎是可行的。
