Department of Chemistry and Key Laboratory of Analytical Sciences of Xiamen University, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Talanta. 2012 May 30;94:356-60. doi: 10.1016/j.talanta.2012.03.046. Epub 2012 Mar 29.
Chitosan, a cationic polysaccharide containing amino and hydroxyl groups, was used to fabricate an electrochemiluminescence (ECL) sensor. In the sensor construction, a glassy carbon electrode (GCE) was first coated by a chitosan film which embedded gold nanoparticles, and then the film was modified by introducing carboxyl groups on the surface, which were used to immobilize tris(2,2'-bipyridyl)ruthenium(II) doped amino-functional silica nanoparticles (NH(2)-RuSiNPs) through amido links. The successful modification was confirmed by scanning electronic microscopy and cyclic voltammetry. A binding model between the chitosan/nanogold composite film and NH(2)-RuSiNPs was also proposed, in which the amido link was the dominant bonding, accompanied with hydrogen bond interaction. ECL studies revealed that the sensor had very good response to different concentrations of 2-(dibutylamino) ethanol. This sensor was also applied in methamphetamine determination.
壳聚糖是一种含有氨基和羟基的阳离子多糖，被用于制备电化学发光（ECL）传感器。在传感器的构建过程中，首先将玻碳电极（GCE）涂覆一层壳聚糖膜，其中嵌入了金纳米粒子，然后通过在表面引入羧基基团对该膜进行修饰，通过酰胺键将三（2,2'-联吡啶）钌（II）掺杂的氨基功能化硅纳米粒子（NH(2)-RuSiNPs）固定在修饰后的电极表面。成功的修饰通过扫描电子显微镜和循环伏安法得到了确认。还提出了壳聚糖/纳米金复合膜与 NH(2)-RuSiNPs 之间的结合模型，其中酰胺键是主要的结合键，同时伴随着氢键相互作用。ECL 研究表明，该传感器对不同浓度的 2-(二丁氨基)乙醇具有很好的响应。该传感器还被应用于甲基苯丙胺的检测。
