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年龄和诊断对人外周血干细胞制品离心及冷冻保存过程中活力的影响。

Impact of age and diagnosis on viability during centrifugation and cryopreservation of peripheral blood stem cell products.

作者信息

Civriz Bozdag S, Bay M, Ayyıldız E, Topcuoglu P, Ilhan O

机构信息

Department of Hematology, Ankara University School of Medicine, Ankara, Turkey.

出版信息

Transfus Apher Sci. 2012 Aug;47(1):117-20. doi: 10.1016/j.transci.2012.05.012. Epub 2012 May 19.

DOI:10.1016/j.transci.2012.05.012
PMID:22609193
Abstract

BACKGROUND

The viability of the hematopoietic stem cells infused to the patient is important for transplant outcome.

STUDY DESIGN AND METHODS

We evaluated 31 peripheral blood stem cell product collected from 15 patients. We aimed to check the viabilities of the cells from patients with different age and diagnosis, in different stages of the cryopreservation procedure.

RESULTS

We showed a markedly decreased viability rate after centrifugation and addition of DMSO. Percentages of viabilities were similar between young and old patients in each step. Type of hematological malignancy did not make a significant influence on the viability.

CONCLUSION

High speed centrifugation has a negative impact on the viability.

摘要

背景

输注给患者的造血干细胞的活力对移植结果很重要。

研究设计与方法

我们评估了从15名患者采集的31份外周血干细胞产品。我们旨在检查不同年龄和诊断的患者在冷冻保存过程不同阶段的细胞活力。

结果

我们发现离心并添加二甲基亚砜（DMSO）后活力率显著降低。每个步骤中年轻患者和老年患者的活力百分比相似。血液系统恶性肿瘤的类型对活力没有显著影响。

结论

高速离心对活力有负面影响。

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