Jun Hyung Jin, Lim Hyun Woo, Choi June, Jung Hak Hyun, Chae Sung Won
Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Ansan hospital, Gyunggi-do, Ansan, South Korea.
Int J Pediatr Otorhinolaryngol. 2012 Aug;76(8):1136-9. doi: 10.1016/j.ijporl.2012.04.017. Epub 2012 May 18.
Peroxisome proliferator activated receptor-γ (PPAR-γ), a member of the nuclear hormone receptor superfamily, plays an important role in the regulation of mucosal inflammation. The aim of this study was to investigate the anti-inflammatory effect of a PPAR-γ agonist, ciglitazone, on cigarette smoke solution (CSS)-induced inflammation in human middle ear epithelial cell lines (HMEECs).
HMEECs with or without ciglitazone pre-treatment were exposed to CSS in order to induce the inflammatory response. The suppressive effect of inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) and cyclooxygenase-2(COX-2), were evaluated using real-time polymerase chain reaction and Western blotting.
Stimulation with CSS at 40 μg/ml for 6 h resulted in a 4.1-fold increase in the expression of TNF-α mRNA in the HMEECs. CSS-induced up-regulation of TNF-α mRNA was decreased by more than 2.8-fold in cells pre-treated with ciglitazone. The up-regulation of COX-2 mRNA and increased COX-2 protein expression induced by CSS were also inhibited by more than 3.7-fold with ciglitazone pre-treatment.
These findings suggest that the inflammatory response induced by CSS could be inhibited by ciglitazone, a PPAR-γ agonist, in HMEECs. As such, PPAR-γ agonists may have therapeutic potential for the treatment of otitis media.
过氧化物酶体增殖物激活受体γ（PPAR-γ）是核激素受体超家族的成员之一，在黏膜炎症调节中起重要作用。本研究旨在探讨PPAR-γ激动剂环格列酮对香烟烟雾溶液（CSS）诱导的人中耳上皮细胞系（HMEECs）炎症的抗炎作用。
对经或未经环格列酮预处理的HMEECs暴露于CSS以诱导炎症反应。使用实时聚合酶链反应和蛋白质印迹法评估炎性细胞因子如肿瘤坏死因子-α（TNF-α）和环氧化酶-2（COX-2）的抑制作用。
用40μg/ml CSS刺激6小时导致HMEECs中TNF-α mRNA表达增加4.1倍。在经环格列酮预处理的细胞中，CSS诱导的TNF-α mRNA上调降低了2.8倍以上。环格列酮预处理也使CSS诱导的COX-2 mRNA上调和COX-2蛋白表达增加受到超过3.7倍的抑制。
这些发现表明，PPAR-γ激动剂环格列酮可抑制HMEECs中CSS诱导的炎症反应。因此，PPAR-γ激动剂可能具有治疗中耳炎的潜力。
