Department of Applied Chemistry, National Defense Academy, Yokosuka 239-8686, Japan.
Cryobiology. 2012 Oct;65(2):113-6. doi: 10.1016/j.cryobiol.2012.05.002. Epub 2012 May 16.
The supercooling and vitrification of aqueous glycerol solutions was studied at high pressures. Homogeneous ice nucleation temperatures (T(H)) were obtained for aqueous glycerol solutions of R=50, 30, 20, 12, and 10 (R: moles of water/moles of glycerol) up to 300MPa. The R=20 glycerol solution formed a glass above 200MPa at a cooling rate of 200°C/min, indicating that pressure enhances glass-formation of aqueous glycerol solutions. The (dT(g)/dP) values were obtained for vitrified aqueous glycerol solutions of R=3, 5, 10, and 20. These data can be used for the development of cryo-preservation liquids for living cells at high pressures.
水甘油溶液的过冷和玻璃化在高压下进行了研究。在 300MPa 以下，获得了 R=50、30、20、12 和 10（R：水的摩尔数/甘油的摩尔数）的水甘油溶液的同质成冰温度（T(H)）。在 200°C/min 的冷却速率下，R=20 的甘油溶液在 200MPa 以上形成了玻璃，表明压力增强了水甘油溶液的玻璃形成。获得了 R=3、5、10 和 20 的玻璃化水甘油溶液的(dT(g)/dP)值。这些数据可用于在高压下开发用于活细胞的冷冻保存液。
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