College of Chemistry, Chemical Engineering and Material Science, Soochow University, Suzhou, Jiangsu 215123, PR China.
Biosens Bioelectron. 2012 Aug-Sep;37(1):88-93. doi: 10.1016/j.bios.2012.04.044. Epub 2012 May 8.
A comparison of the electrochemical and photoelectrochemical behaviors of three biosensors, based on the use of Au, CdS, and ZnS nanoparticles-glucose oxidase (GOD) system, is discussed. All the nanoparticles were electrodeposited onto the indium tin oxide (ITO) thin film coated glass surface. GOD was then immobilized on the nanoparticles-modified electrodes surface with the sol-gel technique. The deposited nanoparticles on ITO electrodes were characterized by scanning electron microscopy, UV-vis spectroscopy and electrochemical impedance spectra. The direct electrochemistry of GOD, analytical performance of glucose calibration curves and the kinetic parameters of the enzyme reaction were compared for all the electrochemical biosensors. Furthermore, the current response of the quantum dots-GOD system biosensor can be increased after illumination. The electrochemical and photoelectrochemical biosensors based on ZnS nanostructures exhibited higher sensitivity than that of Au or CdS nanostructures. Considering ZnS is nontoxic to human and environment, the results suggest that ZnS nanoparticles-GOD system seems to be a promising platform for fabrication of novel electrochemical and photoelectrochemical biosensors.
讨论了基于金、硫化镉和硫化锌纳米粒子-葡萄糖氧化酶（GOD）体系的三种生物传感器的电化学和光电化学行为的比较。所有的纳米粒子都被电沉积到涂有氧化铟锡（ITO）薄膜的玻璃表面上。然后通过溶胶-凝胶技术将 GOD 固定在纳米粒子修饰电极的表面上。ITO 电极上沉积的纳米粒子通过扫描电子显微镜、紫外可见光谱和电化学阻抗谱进行了表征。比较了所有电化学生物传感器的 GOD 的直接电化学、葡萄糖校准曲线的分析性能和酶反应的动力学参数。此外，量子点-GOD 体系生物传感器的电流响应在光照后会增加。基于 ZnS 纳米结构的电化学和光电化学生物传感器的灵敏度高于 Au 或 CdS 纳米结构。考虑到 ZnS 对人和环境无毒，结果表明 ZnS 纳米粒子-GOD 体系似乎是制造新型电化学和光电化学生物传感器的有前途的平台。
