Universidad Autonoma del Estado de Mexico-CIRA, Apdo postal 367, Toluca, CP 50091, Estado de Mexico, Mexico.
Bioresour Technol. 2012 Jul;116:126-32. doi: 10.1016/j.biortech.2012.03.102. Epub 2012 Apr 5.
Currently, the method most used for measuring the maximum specific growth rate (μ(Hmax)) of heterotrophic biomass is by respirometry, using growth batch tests performed at high food/microorganism ratio. No other technique has been suggested, although the former approach was criticized for providing kinetic constants that could be unrepresentative of the original biomass. An alternative method (seed-increments) is proposed, which relies on measuring the initial rates of respiration (r(O2)(_ini)) at different seeding levels, in a single batch, and in the presence of excess readily biodegradable substrate (S(S)). The ASM1-based underlying equations were developed, which showed that μ(Hmax) could be estimated through the slope of the linear function of r(O2)(_ini)·(V(WW)+v(ML)) vs v(ML) (volume of mixed liquor inoculum); V(WW) represent the wastewater volume added. The procedure was tested, being easy to apply; the postulated linearity was constantly observed and the method is claimed to measure the characteristics of the biomass of interest.
目前，用于测量异养生物量最大比特定生长速率 (μ(Hmax)) 的最常用方法是通过呼吸计进行生长批量测试，使用高食物/微生物比例。虽然以前的方法因提供可能与原始生物量不具有代表性的动力学常数而受到批评，但没有提出其他技术。提出了一种替代方法（种粒增量法），该方法依赖于在单个批次中测量不同种粒水平下的初始呼吸速率 (r(O2)(_ini))，并在过量易生物降解底物 (S(S)) 存在的情况下进行测量。基于 ASM1 开发了基础方程，表明可以通过 r(O2)(_ini)·(V(WW)+v(ML)) 与 v(ML)（接种混合液体积）的线性函数的斜率来估算 μ(Hmax)；V(WW) 表示添加的废水体积。该方法经过了测试，易于应用；始终观察到假设的线性关系，并且该方法据称可以测量感兴趣的生物量的特征。
