Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Republic of Korea.
J Colloid Interface Sci. 2012 Aug 1;379(1):27-32. doi: 10.1016/j.jcis.2012.04.055. Epub 2012 Apr 27.
Multifunctional fluorescent and superparamagnetic Fe(3)O(4)/poly(fluorescein O-methacrylate) [Fe(3)O(4)/poly(FMA)] nanoparticles with core/shell structure were synthesized via surface-initiated polymerization. First, polymerizable double bonds were introduced onto the surface of Fe(3)O(4) nanoparticles via ligand exchange and a condensation reaction. A fluorescent monomer, FMA, was then polymerized to the double bonds at the surface via free-radical polymerization, leading to form a fluorescent polymer shell around the superparamagnetic Fe(3)O(4) core. The resultant Fe(3)O(4)/poly(FMA) nanoparticles were characterized by Fourier transform infrared, nuclear magnetic resonance, and X-ray diffraction spectroscopy to confirm the reactions. Transmission electron microscopy images showed that the Fe(3)O(4)/poly(FMA) nanoparticles have a spherical and monodisperse core/shell morphology. Photoluminescence spectroscopy and superconducting quantum interference device magnetometer analyses confirmed that the Fe(3)O(4)/poly(FMA) nanoparticles exhibited fluorescent and superparamagnetic properties, respectively. In addition, we demonstrated the potential bioimaging application of the Fe(3)O(4)/poly(FMA) nanoparticles by visualizing the cellular uptake of the nanoparticles into A549 lung cancer cells.
具有核壳结构的多功能荧光超顺磁性 Fe(3)O(4)/聚（荧光素 O-甲基丙烯酰胺）[Fe(3)O(4)/poly(FMA)]纳米粒子通过表面引发聚合合成。首先，通过配体交换和缩合反应将可聚合的双键引入 Fe(3)O(4)纳米粒子表面。然后，通过自由基聚合将荧光单体 FMA 聚合到表面的双键上，在超顺磁性 Fe(3)O(4 核的周围形成荧光聚合物壳。通过傅里叶变换红外光谱、核磁共振和 X 射线衍射光谱对所得 Fe(3)O(4)/poly(FMA)纳米粒子进行了表征，以确认反应。透射电子显微镜图像表明，Fe(3)O(4)/poly(FMA)纳米粒子具有球形和单分散的核壳形态。荧光光谱和超导量子干涉仪磁强计分析证实，Fe(3)O(4)/poly(FMA)纳米粒子分别表现出荧光和超顺磁性。此外，我们通过可视化纳米粒子进入 A549 肺癌细胞的细胞摄取，证明了 Fe(3)O(4)/poly(FMA)纳米粒子在生物成像中的潜在应用。
