烟酰胺腺嘌呤二核苷酸磷酸氧化酶 p22phox 基因 549C>T 多态性与冠状动脉疾病的关系。

Association of nicotinamide adenine dinucleotide phosphate oxidase p22phox gene 549C>T polymorphism with coronary artery disease.

机构信息

Department of Cardiology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, China.

出版信息

Chin Med J (Engl). 2012 Apr;125(8):1416-9.

Abstract

BACKGROUND

The p22phox is a critical component of the superoxide-generating vascular nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. Several polymorphisms in p22phox gene are studied for their association with cardiovascular diseases. However, no publication is available to assess the relation of 549C > T polymorphism in p22phox gene to coronary artery disease (CAD) risk. This study was to investigate the effect of the p22phox gene 549C > T polymorphism on CAD risk.

METHODS

Hospital-based case-control study was conducted with 297 CAD patients and 343 healthy persons as the control group. Polymerase chain reaction and pyrosequencing using PSQ 96 MA Pyrosequencer (Biotage AB) were used to detect the polymorphisms. Multiple Logistic regression model was used to adjust the potential confounders and to estimate odds ratio (OR) with 95% confidence intervals (CIs).

RESULTS

The observed genotype frequencies of this polymorphism obeyed the Hardy-Weinberg equilibrium in both cases (P = 0.439) and controls (P = 0.668). The frequency of mutant genotypes (TT + CT) in cases (41.08%) was higher than that in controls (36.73%) with an OR = 1.20 (95%CI = 0.87-1.65). After the adjustment of the potential confounders, there was a significant association of the mutant genotypes with increased risk of CAD (OR = 1.57, 95%CI = 1.01-2.46, P = 0.047).

CONCLUSIONS

The mutant genotypes of the p22phox gene 549C > T polymorphism had a significant effect on the increased risk of CAD in this studied population.

摘要

背景

p22phox 是生成超氧化物的血管烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶的关键组成部分。已有研究探讨了 p22phox 基因中的多种多态性与心血管疾病的关联。然而，目前尚无研究评估 p22phox 基因 549C>T 多态性与冠心病（CAD）风险的关系。本研究旨在探讨 p22phox 基因 549C>T 多态性对 CAD 风险的影响。

方法

采用基于医院的病例对照研究，纳入 297 例 CAD 患者和 343 名健康对照者。采用聚合酶链反应和焦磷酸测序法（PSQ 96 MA Pyrosequencer，Biotage AB）检测该多态性。采用多因素 Logistic 回归模型调整潜在混杂因素，并估计比值比（OR）及其 95%置信区间（CI）。

结果

该多态性的观察基因型频率在病例组（P=0.439）和对照组（P=0.668）均符合 Hardy-Weinberg 平衡。病例组（41.08%）的突变基因型（TT+CT）频率高于对照组（36.73%），OR=1.20（95%CI：0.87-1.65）。调整潜在混杂因素后，突变基因型与 CAD 风险增加显著相关（OR=1.57，95%CI：1.01-2.46，P=0.047）。