Biomolecular Function Research Branch, Division of Convergence Technology, Research Institute, National Cancer Center, Goyang, Gyeonggi, Republic of Korea.
FEBS Lett. 2012 May 7;586(9):1384-8. doi: 10.1016/j.febslet.2012.04.004. Epub 2012 Apr 13.
Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.
古生球菌 Pyrococcus furiosus PF2050 是一种尚未被充分阐明的假定蛋白，它含有两个 DUF2666 结构域。之前尚未对 PF2050 的功能和结构进行研究。在本研究中，我们确定了 PF2050 的晶体结构。PF2050 的结构表明，这两个 DUF2666 结构域紧密相互作用，形成一个球形结构。每个 DUF2666 结构域由一个反平行的 β-折叠和一个 α-螺旋束组成。PF2050 结构的一侧具有带正电荷的碱性裂缝，可能具有 DNA 结合功能。此外，我们证实 PF2050 与圆形和线性 dsDNA 相互作用。
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