采用改进方法对基于肌球蛋白重链IIb、II d和IIa并具有特定碱性轻链组合的快速同工型肌球蛋白进行电泳分离。

Electrophoretic separation by an improved method of fast myosin HCIIb-, HCIId-, and HCIIa-based isomyosins with specific alkali light chain combinations.

作者信息

Termin A, Pette D

机构信息

Fakultät für Biologie, Universität Konstanz, FRG.

出版信息

FEBS Lett. 1990 Nov 26;275(1-2):165-7. doi: 10.1016/0014-5793(90)81463-x.

DOI:10.1016/0014-5793(90)81463-x

PMID:2261985

Abstract

An improved method of electrophoresis under nondenaturing conditions separated three electrophoretically distinct isomyosin triplets when applied to rat fast-twitch muscles displaying a predominance of one of the fast myosin heavy chain isoforms HCIIb, HCIId or HCIIa. The three isomyosin triplets, named FM1b-FM3b, FM1d-FM3d, FM1a-FM3a, corresponded to the three possible alkali light chain (LC) combinations (LC1f homodimer, LC1f/LC3f heterodimer, and LC3f homodimer) with each fast HC isoform. Different proportions of these various isomyosins suggested specific affinities of light chains LC1f and LC3f for the fast heavy chain isoforms.

摘要

一种改进的非变性条件下的电泳方法，应用于以快肌球蛋白重链异构体HCIIb、HCIId或HCIIa之一为主的大鼠快肌时，分离出了三个电泳性质不同的同肌球蛋白三联体。这三个同肌球蛋白三联体，分别命名为FM1b - FM3b、FM1d - FM3d、FM1a - FM3a，对应于每种快肌重链异构体与三种可能的碱性轻链（LC）组合（LC1f同二聚体、LC1f/LC3f异二聚体和LC3f同二聚体）。这些不同同肌球蛋白的不同比例表明轻链LC1f和LC3f对快重链异构体具有特定亲和力。