Peres A
Dip. di Fisiologia e Biochimica Gen. dell'Universitá di Milano, Italy.
FEBS Lett. 1990 Nov 26;275(1-2):213-6. doi: 10.1016/0014-5793(90)81474-3.
To better understand the mechanism of intracellular Ca2+ mobilization, mouse oocytes were micro-injected with 'caged'-inositol-1,4,5 triphosphate caged-InsP3) together with the Ca2+ indicator Fluo-3 to directly induce and monitor Ca2+ redistribution. Photo-released InsP3 elicits [Ca2+]i changes exhibiting several kinetic phases and threshold behaviour. Often Ca2+ oscillations were induced after a single InsP3 pulse. Autoregenerative Ca2+ transients could also be induced by injections of Ca2+ itself, demonstrating unequivocally the presence of a Ca2(+)-induced Ca2(+)-release mechanism in these cells.
为了更好地理解细胞内钙离子动员的机制，将“笼锁”肌醇-1,4,5-三磷酸（笼锁肌醇三磷酸）与钙离子指示剂Fluo-3一起显微注射到小鼠卵母细胞中，以直接诱导并监测钙离子的重新分布。光释放的肌醇三磷酸引发细胞内钙离子浓度（[Ca2+]i）变化，呈现出几个动力学阶段和阈值行为。单次肌醇三磷酸脉冲后常诱导出钙离子振荡。注射钙离子本身也可诱导出自发性钙离子瞬变，明确证明这些细胞中存在钙离子诱导的钙离子释放机制。
