Morley P, Calaresu F R, Armstrong D T
Department of Physiology, University of Western Ontario, London, Canada.
FEBS Lett. 1990 Nov 26;275(1-2):70-2. doi: 10.1016/0014-5793(90)81441-p.
The ovaries of many species contain catecholamines and beta-adrenergic receptors. The present studies were done to determine if catecholamines play a role in the regulation of androgen production by porcine theca cells. Basal and luteinizing hormone (LH)-stimulated androstenedione production was significantly inhibited by noradrenaline and isoproterenol. The inhibitory effects were dose-dependent and were enhanced when the cultures contained the carboxy-O-methyl transferase inhibitor, U-0521. The inhibitory effect of isoproterenol was reversed by the beta-adrenergic antagonist, metoprolol. Isoproterenol caused a generalized inhibition of LH-stimulated steroidogenesis, decreasing the accumulation of pregnenolone, progesterone, androstenedione and estradiol in the culture medium. These studies suggest that catecholamines may be important regulators of thecal androgen production.
许多物种的卵巢都含有儿茶酚胺和β-肾上腺素能受体。本研究旨在确定儿茶酚胺是否在猪卵泡膜细胞雄激素生成的调节中发挥作用。去甲肾上腺素和异丙肾上腺素显著抑制基础及促黄体生成素（LH）刺激的雄烯二酮生成。抑制作用呈剂量依赖性，当培养物中含有羧基-O-甲基转移酶抑制剂U-0521时，抑制作用增强。β-肾上腺素能拮抗剂美托洛尔可逆转异丙肾上腺素的抑制作用。异丙肾上腺素对LH刺激的类固醇生成产生广泛抑制，减少培养基中孕烯醇酮、孕酮、雄烯二酮和雌二醇的积累。这些研究表明，儿茶酚胺可能是卵泡膜雄激素生成的重要调节因子。
