促黄体生成激素释放激素降解活性在下丘脑的亚细胞定位。

Subcellular localization of luteinizing hormone releasing hormone degrading activity in the hypothalamus.

作者信息

Parker C R, Foreman M M, Porter J C

出版信息

Brain Res. 1979 Oct 5;174(2):221-8. doi: 10.1016/0006-8993(79)90846-1.

Abstract

The in vitro degradation of endogenous as well as exogenous luteinizing hormone releasing hormone (LHRH) by subcellular fractions of rat hypothalamic tissue was studied. Endogenous LHRH, localized an isolated nerve terminals (synaptosomes), was found to be resistant to enzymatic degradation (60 min, 37 degrees C) as long as the synaptosomal membrane remained intact. Endogenous LHRH was rapidly degraded by the 900 x g supernatant fluid and cytosol but not by myelin/microsomes, intact synaptosomes, or mitochondria. Lysed synaptosomes rapidly degraded exogenous LHRH. The LHRH degrading activity of synaptosomes was highly concentrated in the 'synaptosol', i.e., the cytosol of the nerve terminal. These data suggest that the LHRH degrading activity of the rat hypothalamus is a readily solubilized component of neurons, and possibly of non-neuronal cells.

摘要

研究了大鼠下丘脑组织亚细胞组分对内源性及外源性促黄体生成素释放激素（LHRH）的体外降解情况。内源性LHRH定位于分离的神经末梢（突触体），只要突触体膜保持完整，就发现其对酶降解具有抗性（60分钟，37℃）。内源性LHRH可被900×g上清液和胞质溶胶快速降解，但不能被髓磷脂/微粒体、完整的突触体或线粒体降解。裂解的突触体可快速降解外源性LHRH。突触体的LHRH降解活性高度集中在“突触溶胶”中，即神经末梢的胞质溶胶。这些数据表明，大鼠下丘脑的LHRH降解活性是神经元以及可能是非神经元细胞中一种易于溶解的成分。