Tytell M, Clark J H, Peck E J
Neurochem Res. 1980 May;5(5):493-504. doi: 10.1007/BF00964986.
Mitochondrial-synaptosomal fractions (P2) from the basomedial hypothalamus of adult ovariectomized rats were employed to study the effects of estradiol benzoate (EB) and progesterone (P) on the release of luteinizing hormone-releasing hormone (LHRH). Treatment of ovariectomized rats with 5 or 50 micrograms of EB significantly reduced the total LHRH released from P2 under both control and K+-stimulated conditions. Furthermore, rats given 50 microgram EB demonstrated cyclic variations in the magnitude of inhibition of LHRH release. Comparison of LHRH release from P2 of rats sacrificed at 0900 hr with that from those sacrificed at 1500 hr revealed a small but persistent facilitation of LHRH release each afternoon. This facilitation, associated with an increase in the soluble component of LHRH release, was absent when rats also received 5 mg of P. No effects on LHRH release were observed when 17 beta-estradiol alone or when P was applied to P2 in vitro. The data show that the regulatory effects of estrogen and progesterone given in vivo on LHRH secretion can be observed in a subcellular fraction of the hypothalamus containing neurosecretory cell terminals.
采用成年去卵巢大鼠基底内侧下丘脑的线粒体 - 突触体组分（P2）来研究苯甲酸雌二醇（EB）和孕酮（P）对促黄体生成激素释放激素（LHRH）释放的影响。用5或50微克EB处理去卵巢大鼠，在对照和钾离子刺激条件下，均显著降低了从P2释放的总LHRH。此外，给予50微克EB的大鼠在LHRH释放抑制程度上表现出周期性变化。比较上午9点处死的大鼠P2中LHRH的释放与下午3点处死的大鼠P2中LHRH的释放，发现每天下午LHRH释放有轻微但持续的促进作用。当大鼠同时接受5毫克P时，这种与LHRH释放可溶性成分增加相关的促进作用消失。单独使用17β - 雌二醇或在体外将P应用于P2时，未观察到对LHRH释放的影响。数据表明，体内给予雌激素和孕酮对LHRH分泌的调节作用可在含有神经分泌细胞终末的下丘脑亚细胞组分中观察到。
