Comparative evaluation of three Lactobacilli with strain-specific activities for rats when supplied in drinking water.

To test the in vivo benefits of three lactobacilli and to compare their different efficacies based on strain-specific activities by using rats as an experimental model, a growth-promotion and a challenge trial were conducted. The three strains, Lactobacillus salivarius G1-1, Lactobacillus reuteri G22-2, and Lactobacillus reuteri G8-5 shared antimicrobial, bile-salt-hydrolase and amylolytic activities in vitro, respectively. In the 17 day growth-promotion trial, 48 rats were allotted to four treatments with 12 replicates per treatment: a control group, which received saline, as well as three experimental groups, which received 10(8) cells/ml of one of the three lactobacilli in saline suspension. The results showed that compared with the control group, L. reuteri G8-5 significantly improved feed efficiency and decreased fecal pH values on days 8 and 17, concomitant with reduced faecal coliform counts on d 17 (p < 0.05). All treatments with lactobacilli caused an increase in the moisture content of the faeces and a decrease in the serum total cholesterol and blood urea nitrogen levels. High-density lipoprotein cholesterol was only elevated for rats which received L. reuteri G22-2. In the Salmonella-challenge trial, 40 rats were allotted to five treatments (8 replicates per treatment) which consisted of a positive control (infected, no Lactobacillus pretreatment), a negative control (uninfected, no Lactobacillus pretreatment) and three Lactobacillus-pretreated groups (10(9) cells/ml in saline). The results showed that rats in all Lactobacillus pretreated groups were protected from infection with significantly higher weight gain, feed intake and feed efficiency compared with positive control rats (p < 0.05). Rats treated with L. salivarius G1-1 and L. reuteri G22-2 tended to exhibit higher weight gains than those pretreated with L. reuteri G8-5. Significantly lower Salmonella shedding in faeces, Salmonella numbers in the spleen and the relative weight of the spleen were observed in the Lactobacillus groups (p < 0.05). Based on the overall results, it can be concluded that not all strains within the same lactobacilli species show similar effects and that some of the beneficial functionalities to animals were strain-specific. Therefore strains for practical application need to be carefully selected based on their strain-specific characters.