Su Yating, Chen Xingjie, Liu Ming, Guo Xiaohua
Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities, No. 182, Minyuan Road, Hongshan District, Wuhan, Hubei Province 430074 China.
Guangxi Yang-Xiang Animal Husbandry Co. Ltd., Guigang, Guangxi Province 537100 China.
J Anim Sci Biotechnol. 2017 Jun 9;8:52. doi: 10.1186/s40104-017-0183-3. eCollection 2017.
The beneficial effects of probiotics in animal production are often strain-related. Different strains from the same species may exert different weight-gain effect on hosts in vivo. Most lactobacilli are selected based on their in vitro activities, and their metabolism and regulation on the intestine based on strain-related characters are largely unexplored. The objective of the present study was to study the in vivo effects of the three lactobacilli on growth performance and to compare the differential effects of the strains on the faecal microbiota and ileum mucosa proteomics of piglets.
Three hundred and sixty piglets were assigned to one of four treatments, which included an antibiotics-treated control and three experimental groups supplemented with the three lactobacilli, G1-1, G8-5 and G22-2, respectively. Piglets were weighed and the feed intake was recorded to compare the growth performance. The faecal lactobacilli and coliform was quantified using quantitative PCR and the faecal microbiota was profiled by denaturing gradient gel electrophoresis (DGGE). The proteomic approach was applied to compare the differential expression of proteins in the ileum mucosa.
No statistical difference was found among the three -treated groups in animal growth performance compared with the antibiotics-treated group ( > 0.05). Supplementation of lactobacilli in diets significantly increased the relative 16S rRNA gene copies of genus on both d 14 and d 28 ( < 0.05)., and the bacterial community profiles based on DGGE from the lactobacilli-treated groups were distinctly different from the antibiotics-treated group ( < 0.05). The ileum mucosa of piglets responded to all supplementation by producing more newly expressed proteins and the identified proteins were all associated with the functions beneficial for stabilization of cell structure. Besides, some other up-regulated and down-regulated proteins in different -treated groups showed the expression of proteins were partly strain-related.
All the three lactobacilli in this study show comparable effects to antibiotics on piglets growth performance. The three lactobacilli were found able to modify intestinal microbiota and mucosa proteomics. The regulation of protein expression in the intestinal mucosa are partly associated with the strains administrated in feed.
益生菌在动物生产中的有益作用通常与菌株相关。同一物种的不同菌株对宿主体内的体重增加效应可能不同。大多数乳酸杆菌是根据其体外活性选择的，基于菌株相关特性对肠道的代谢和调节作用在很大程度上尚未得到探索。本研究的目的是研究三种乳酸杆菌对生长性能的体内影响，并比较这些菌株对仔猪粪便微生物群和回肠黏膜蛋白质组学的差异影响。
360头仔猪被分配到四种处理之一，其中包括抗生素处理的对照组和分别添加三种乳酸杆菌G1-1、G8-5和G22-2的三个实验组。对仔猪进行称重并记录采食量，以比较生长性能。使用定量PCR对粪便中的乳酸杆菌和大肠菌群进行定量，并通过变性梯度凝胶电泳（DGGE）分析粪便微生物群。应用蛋白质组学方法比较回肠黏膜中蛋白质的差异表达。
与抗生素处理组相比，三个处理组之间在动物生长性能方面未发现统计学差异（P＞0.05）。在日粮中添加乳酸杆菌在第14天和第28天均显著增加了属的相对16S rRNA基因拷贝数（P＜0.05），并且基于DGGE的乳酸杆菌处理组的细菌群落图谱与抗生素处理组明显不同（P＜0.05）。仔猪回肠黏膜对所有添加物的反应是产生更多新表达的蛋白质且鉴定出的蛋白质均与有利于细胞结构稳定的功能相关。此外，不同处理组中一些其他上调和下调的蛋白质表明蛋白质表达部分与菌株相关。
本研究中的三种乳酸杆菌在仔猪生长性能方面显示出与抗生素相当的效果。发现这三种乳酸杆菌能够改变肠道微生物群和黏膜蛋白质组学。肠道黏膜中蛋白质表达的调节部分与饲料中施用的菌株有关。
