Department of Oral Biology, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
J Periodontol. 2013 Apr;84(4):529-34. doi: 10.1902/jop.2012.120188. Epub 2012 May 25.
Increased collagenolytic activity, characteristic of uncontrolled diabetes, may compromise collagen membrane (CM) survival. Tetracycline (TCN) possesses anticollagenolytic properties and delays CM degradation in healthy animals. This study evaluates the degradation of TCN--immersed and -non-immersed CMs in rats with diabetes compared to those with normoglycemia.
Diabetes was induced in 15 12-week-old male Wistar rats by injection of 65 mg/kg streptozotocin. The control group consisted of 15 rats with normoglycemia. Sixty bilayered CM disks were labeled before implantation with aminohexanoyl-biotin-N-hydroxy-succinimide ester, of which 30 were immersed in 50 mg/mL TCN solution (experimental) or phosphate-buffered saline (PBS) (control). In each animal, two disks (control and experimental) were implanted in two midsagittal calvarial defects in the parietal bone. Similar non-implanted disks served as baseline. After 3 weeks, animals were euthanized, and the calvaria and overlying soft tissues were processed for demineralized histologic analysis. Horseradish peroxidase-conjugated streptavidin was used to detect the biotinylated collagen. The area of residual collagen within the membrane disks was measured and analyzed with a digital image analysis system. Several slides from each specimen were also stained with hematoxylin and eosin. Statistical analysis consisted of paired and unpaired t tests.
The amount of residual collagen in PBS-immersed disks was lower in rats with diabetes compared to rats with normoglycemia (69% of baseline versus 93%, respectively, P <0.001). TCN immersion increased the amount of residual collagen contents in both diabetic (83% of baseline) and healthy (97.5% of baseline) animals (P <0.0001).
Diabetes increases CM degradation, whereas immersion in 50 mg/mL TCN solution before implantation presents an opposite effect.
不受控制的糖尿病会导致胶原蛋白分解活性增加，从而可能影响胶原膜（CM）的存活。四环素（TCN）具有抗胶原分解作用，并能延缓健康动物 CM 的降解。本研究评估了糖尿病大鼠与正常血糖大鼠中 TC 浸泡和非浸泡 CM 的降解情况。
通过注射 65mg/kg 链脲佐菌素诱导 15 只 12 周龄雄性 Wistar 大鼠糖尿病。对照组由 15 只血糖正常的大鼠组成。在植入前，将 60 个双层 CM 圆盘用氨己酰基生物素-N-羟基琥珀酰亚胺酯标记，其中 30 个浸泡在 50mg/mL TCN 溶液（实验组）或磷酸盐缓冲盐水（PBS）（对照组）中。在每只动物中，两块圆盘（对照和实验）分别植入顶骨中线两侧的两个颅骨缺损中。类似的未植入圆盘作为基线。3 周后，处死动物，将颅骨和覆盖的软组织进行脱钙组织学分析。辣根过氧化物酶偶联的链霉亲和素用于检测生物素化的胶原蛋白。用数字图像分析系统测量和分析膜盘内残留胶原蛋白的面积。每个标本的几张切片还进行了苏木精和伊红染色。统计分析包括配对和非配对 t 检验。
与血糖正常的大鼠相比，PBS 浸泡的糖尿病大鼠 CM 中残留胶原蛋白的量减少（分别为基线的 69%和 93%，P<0.001）。TCN 浸泡增加了糖尿病（基线的 83%）和健康（基线的 97.5%）大鼠中残留胶原蛋白含量（P<0.0001）。
糖尿病会增加 CM 降解，而植入前浸泡在 50mg/mL TCN 溶液中则会产生相反的效果。
