Tal Haim, Weinreb Miron, Shely Asaf, Nemcovsky Carlos E, Moses Ofer
Department of Periodontology and Dental Implantology, Tel Aviv University School of Dental Medicine, Ramat Aviv, Tel Aviv, 69978, Israel.
Department of Oral Biology, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
Clin Oral Investig. 2016 Jul;20(6):1237-42. doi: 10.1007/s00784-015-1615-0. Epub 2015 Oct 8.
The present study evaluated the degradation of collagen matrix (CM) immersed in tetracycline (TTC) or phosphate-buffered saline (PBS) in diabetic and normoglycemic rats.
Diabetes was induced in 15 rats by systemic streptozotocin (STZ) (experimental); 15 healthy rats served as controls. One day before implantation 60 CM disks, 5 mm in diameter, were labeled with biotin: 30 were immersed in tetracycline (TTC) and 30 in PBS. One disk of each type was implanted subdermally in each rat. Animals were euthanized after 3 weeks, and tissue specimens containing the disks were prepared for histologic analysis. Horseradish peroxidase (HRP)-conjugated streptavidin was used to detect the remaining biotinylated collagen. Residual collagen area within the CM disks was analyzed and compared to baseline.
Diabetes significantly increased the CM degradation. Immersion of the CM disks in a 50-mg/mL TTC solution before implantation decreased its degradation both in diabetic and normoglycemic rats.
Diabetes significantly increases collagen matrix degradation; immersion of collagen matrix in TTC before implantation decreases its degradation in both diabetic and normoglycemic conditions.
Immersion of medical collagen devices in TTC may be an effective means to decrease their resorption rate and increase their effectiveness, especially in situations with increased degradation such as diabetes.
本研究评估了糖尿病大鼠和血糖正常大鼠体内浸泡于四环素（TTC）或磷酸盐缓冲盐水（PBS）中的胶原基质（CM）的降解情况。
通过全身注射链脲佐菌素（STZ）诱导15只大鼠患糖尿病（实验组）；15只健康大鼠作为对照。在植入前一天，将60个直径5毫米的CM盘用生物素标记：30个浸泡于四环素（TTC）中，30个浸泡于PBS中。每种类型的一个盘皮下植入每只大鼠体内。3周后对动物实施安乐死，并制备包含圆盘的组织标本用于组织学分析。用辣根过氧化物酶（HRP）偶联的链霉亲和素检测剩余的生物素化胶原。分析CM盘内的残余胶原面积并与基线进行比较。
糖尿病显著增加了CM的降解。植入前将CM盘浸泡于50毫克/毫升的TTC溶液中可降低其在糖尿病大鼠和血糖正常大鼠体内的降解。
糖尿病显著增加胶原基质降解；植入前将胶原基质浸泡于TTC中可降低其在糖尿病和血糖正常情况下的降解。
将医用胶原装置浸泡于TTC中可能是降低其吸收速率并提高其有效性的有效方法，尤其是在诸如糖尿病等降解增加的情况下。
