Department of Microbiology, Kasturba Medical College, Manipal University, Manipal 576104, India.
J Infect Public Health. 2012 Jun;5(3):257-62. doi: 10.1016/j.jiph.2012.03.004. Epub 2012 Apr 30.
The objective of this study was to evaluate the microbial quality of the well water used as a drinking source in urban and rural households.
A total of 80 household well water samples were analyzed by the multiple fermentation tube method to determine the presumptive coliform count/most probable number of coliforms, and the isolates were identified using standard procedures, followed by susceptibility testing.
Fecal indicator organisms, including Escherichia coli and Enterococcus spp. were isolated from 22 (27.5%) samples, and the majority (92.5%) of the water sources were contaminated with coliforms. A total of 170 bacterial isolates were obtained, including coliforms (70%), Enterococcus spp. (1.8%) and saprophytes (28.2%). A significant number of isolates were multi-drug resistant, which is a cause of concern. A comparison of the microbial quality of the water between urban and rural households revealed no significant differences.
It might be prudent to monitor the bacteriological quality of well water at the source in addition to resistance profiles of the isolates.
本研究旨在评估城乡家庭饮用水源井水的微生物质量。
采用多管发酵法对 80 户家庭井水水样进行分析,以确定大肠菌群的可能数/大肠菌群的最可然数,并采用标准程序对分离株进行鉴定和药敏试验。
从 22 份(27.5%)水样中分离出粪便指示菌,包括大肠杆菌和肠球菌属,其中大多数(92.5%)水源受到大肠菌群污染。共获得 170 株细菌分离株,包括大肠菌群(70%)、肠球菌属(1.8%)和腐生菌(28.2%)。相当数量的分离株具有多重耐药性,这令人担忧。城乡家庭之间的水样微生物质量比较无显著差异。
除了分离株的耐药谱外,可能还需要谨慎监测井水的细菌学质量。