硒盐对 DNA 结构的影响。

The effect of Se salts on DNA structure.

机构信息

Department of Chemistry, Islamic Azad University, Central Tehran Branch (IAUCTB), Tehran 14676-86831, Iran.

出版信息

J Photochem Photobiol B. 2012 Aug 1;113:36-41. doi: 10.1016/j.jphotobiol.2012.04.012. Epub 2012 May 8.

DOI:10.1016/j.jphotobiol.2012.04.012

Abstract

There is considerable interest in the role of selenium in cancer prevention. Various organic and inorganic Se compounds are considered to be antioxidants. In the present study, the binding modes, the binding constants and the stability of Se-DNA complexes have been determined by Fourier transform infrared (FTIR) and UV-Visible spectroscopic methods. Spectroscopic evidence showed that Na(2)SeO(4) and Na(2)SeO(3) bind to the minor and major grooves of DNA and the backbone phosphate (PO(2)) with overall binding constants of K(Na(2)SeO(4)-DNA)=5.20×10(4) M(-1) and K(Na(2)SeO(3)-DNA)=1.87×10(3) M(-1). DNA aggregations occurred at high selenium concentrations. No biopolymer conformational changes were observed upon Na(2)SeO(3) and Na(2)SeO(4) interactions, while DNA remained in the B-family structure.

摘要

人们对硒在癌症预防中的作用非常感兴趣。各种有机和无机硒化合物被认为是抗氧化剂。在本研究中，通过傅里叶变换红外（FTIR）和紫外可见光谱法确定了硒-DNA 配合物的结合模式、结合常数和稳定性。光谱证据表明，Na2SeO4 和 Na2SeO3 与 DNA 的小沟和大沟以及骨架磷酸（PO2）结合，总结合常数为 K（Na2SeO4-DNA）=5.20×104 M-1 和 K（Na2SeO3-DNA）=1.87×103 M-1。在高硒浓度下会发生 DNA 聚集。Na2SeO3 和 Na2SeO4 相互作用时，没有观察到生物聚合物构象发生变化，而 DNA 仍保持 B 族结构。