Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
J Proteomics. 2012 Jul 16;75(13):3855-65. doi: 10.1016/j.jprot.2012.04.051. Epub 2012 May 23.
The trochophore and D-veliger are two pivotal but distinct developmental stages in bivalve larval development. Complex morphological and physiological processes are involved in the transition between these stages, yet the underlying molecular mechanisms remain largely elusive. In this study, a comparative proteomic analysis was conducted to identify the proteins related to early larval development of the Pacific oyster Crassostrea gigas. Fifty proteins showing different levels of expression were screened. These spots were further analyzed using LC-MS/MS and twenty of them were identified. Together with information from Western blotting and real-time PCR assay of some annotated genes, our results provide insight into novel aspects of cellular processes and protein function in oyster larvae. For example, it was shown that cell division was unexpectedly slowed down in D-veliger larvae, while substantial gene transcription and biochemical activity were revealed. "Quality control" proteins were identified for the first time to be involved in mollusk larval development. Bivalve larval development provides an elegant system for studying cellular processes and tissue morphogenesis. Our data shed light on the molecular mechanisms underlying these complex processes and regulatory networks.
担轮幼虫和 D-型幼虫是双壳类幼虫发育过程中的两个关键但不同的发育阶段。在这些阶段之间的转换涉及复杂的形态和生理过程，但潜在的分子机制在很大程度上仍未被揭示。在这项研究中，进行了比较蛋白质组学分析，以鉴定太平洋牡蛎（Crassostrea gigas）早期幼虫发育相关的蛋白质。筛选出了 50 种表达水平不同的蛋白质。使用 LC-MS/MS 对这些斑点进行了进一步分析，鉴定出了其中的 20 种。结合一些注释基因的 Western blot 和实时 PCR 分析的信息，我们的结果提供了对牡蛎幼虫细胞过程和蛋白质功能的新认识。例如，表明 D-型幼虫中的细胞分裂出乎意料地减慢，而大量的基因转录和生化活性被揭示。首次鉴定到“质量控制”蛋白质参与软体动物幼虫发育。双壳类幼虫发育为研究细胞过程和组织形态发生提供了一个优雅的系统。我们的数据阐明了这些复杂过程和调控网络背后的分子机制。
