Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
Microbes Infect. 2012 Sep;14(11):922-9. doi: 10.1016/j.micinf.2012.05.003. Epub 2012 May 22.
The Ca(2+)-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoH(V83F), was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoH(V83F) mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH.
钙调磷酸酶途径影响丝状真菌的毒力和形态发生。在这里，我们鉴定了 37 种与烟曲霉中钙调磷酸酶（CalA）的催化亚基相互作用的 CalA 相互作用蛋白，包括核苷二磷酸激酶（SwoH）。通过双分子荧光互补验证了 CalA 和 SwoH 之间的体内相互作用。A. fumigatus swoH 是一个必需基因。因此，构建了一个在活性位点发生点突变的温度敏感条件突变株 SwoH(V83F)，该突变株表现出生长减慢和对高温的敏感性增加。SwoH(V83F)突变不会导致在大蜡螟感染模型中丧失毒力。这些结果表明 CalA 与 SwoH 相互作用。
