Arizono Naoki, Kuramochi Toshiaki, Kagei Noboru
Department of Infectious Diseases, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Parasitol Int. 2012 Dec;61(4):715-8. doi: 10.1016/j.parint.2012.05.011. Epub 2012 May 24.
Acanthocephalans of the genus Bolbosoma are intestinal parasites of marine mammals with a lifecycle similar to that of anisakid nematodes. Several cases of Bolbosoma infection in humans have been reported, but no species identification has been made. Here, we report a case of Bolbosoma infection, in which the worm was found in histological sections of the partially resected small intestine of a Japanese man. Morphological features of the worm reconstructed from serial sectioning indicated that the worm was most likely to be a sexually immature female of Bolbosoma capitatum. DNA extraction from paraffin-embedded sections and ITS1-5.8S rRNA-ITS2 sequencing showed that this species formed a monophyletic group with Bolbosoma nipponicum, and was clearly distinguishable from Corynosoma spp. or Polymorphus spp. These results may provide a reference for identifying and characterizing unknown acanthocephalans found in histological sections.
泡吻棘头虫属的棘头虫是海洋哺乳动物的肠道寄生虫，其生命周期与异尖线虫相似。已有数例人类感染泡吻棘头虫的病例报道，但尚未进行物种鉴定。在此，我们报告一例泡吻棘头虫感染病例，该虫是在一名日本男性部分切除的小肠组织切片中发现的。通过连续切片重建的虫体形态特征表明，该虫很可能是头状泡吻棘头虫的未成熟雌虫。从石蜡包埋切片中提取DNA并进行ITS1-5.8S rRNA-ITS2测序显示，该物种与日本泡吻棘头虫形成一个单系群，且与冠棘头虫属或多形棘头虫属明显不同。这些结果可为鉴定和表征组织切片中发现的未知棘头虫提供参考。
