Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.
Cancer Lett. 2012 Nov 28;324(2):186-96. doi: 10.1016/j.canlet.2012.05.022. Epub 2012 May 22.
MicroRNAs (miRNAs) play an important role in cancer initiation, progression and metastasis by regulating their target genes. Here, we found microRNA-10a (miR-10a) is upregulated in human cervical cancer and promotes the colony formation activity, migration and invasion of HeLa and C33A cells. Subsequently, CHL1 is confirmed as a target of miR-10a and is negatively regulated by miR-10a at mRNA and protein levels. Furthermore, knockdown of CHL1 expression results in increased colony formation activity, migration and invasion. Finally, overexpression of CHL1 lacked the 3'UTR abolished the effects of miR-10a. Our results may provide a strategy for blocking tumor metastasis.
微小 RNA（miRNAs）通过调控靶基因在癌症的发生、发展和转移中发挥着重要作用。在这里，我们发现 miR-10a 在人宫颈癌中呈上调表达，并促进 HeLa 和 C33A 细胞的集落形成活性、迁移和侵袭。随后，CHL1 被确认为 miR-10a 的靶基因，并在 mRNA 和蛋白水平上受到 miR-10a 的负调控。此外，敲低 CHL1 的表达导致集落形成活性、迁移和侵袭增加。最后，过表达缺乏 3'UTR 的 CHL1 则消除了 miR-10a 的作用。我们的研究结果可能为阻断肿瘤转移提供了一种策略。
