Institute of Laboratory Medicine, University of Pécs, Pécs H-7624, Hungary.
Int J Biol Macromol. 2012 Oct;51(3):279-83. doi: 10.1016/j.ijbiomac.2012.05.019. Epub 2012 May 23.
The mycotoxin Ochratoxin A (OTA) appears worldwide in cereals, plant products, different foods and drinks. Ochratoxin A binds to plasma albumin with a very high affinity. However, it is well known that natural flavonoids can also bind to human serum albumin (HSA) at the same binding site as OTA does (site I, subdomain IIA). A few experimental literature data suggest that reducing the bound fraction of OTA speeds up its elimination rate with a potential decrease in its toxicity. In our experimental model competitive binding properties of flavonoid aglycones were examined with a fluorescence polarization based approach. Our data show that some of the flavonoids are able to remove the toxin from HSA. We conclude that among the 13 studied flavonoid aglycones galangin and quercetin were the most effective competitors for OTA.
真菌毒素赭曲霉毒素 A（OTA）在世界各地的谷物、植物产品、各种食品和饮料中均有出现。OTA 与血浆白蛋白具有非常高的亲和力。然而，众所周知，天然类黄酮也可以与人体血清白蛋白（HSA）在与 OTA 相同的结合位点（I 位点，IIA 亚域）结合。一些实验文献数据表明，降低 OTA 的结合分数会加速其消除速度，并可能降低其毒性。在我们的实验模型中，使用基于荧光偏振的方法研究了类黄酮苷元的竞争性结合特性。我们的数据表明，一些类黄酮能够将毒素从 HSA 中去除。我们得出结论，在所研究的 13 种类黄酮苷元中，根皮素和槲皮素是 OTA 的最有效竞争物。
