Menna J H, Hankins W D, Kost T, Nolop K, Krantz S B
Exp Hematol. 1979 Jul;7(6):315-23.
A cell culture method has been developed in which spleen cells from Friend virus (FV) infected mice can be studied for virus production as well as erythroid differentiation. Primary spleen cell cultures from plethoric Balb/c mice were initiated at 24, 48 or 73 h after FV infection. These cells manifested a well-defined wave of heme synthesis at approximately 64, 48, or 23 h, respectively, of cell culture. Assays for spleen focus-forming virus (SFFV) and helper murine leukemia virus (MuLV-F) production in these cultures revealed that the peak rates of production of both viruses occurred at essentially the same time as the peaks of heme synthesis. The time at which the peaks of virus production and heme synthesis occurred in vitro was related to the time interval after infection (80-105 h) rather than the time at which the cells were placed in cell culture or the number of hours of cell culture. Medium change experiments suggested that the temporal relation between heme synthesis and virus production was an intrinsic feature of FVP infected cells in this in vitro system.
已开发出一种细胞培养方法，利用该方法可研究感染弗氏病毒（FV）小鼠的脾细胞的病毒产生情况以及红系分化情况。在FV感染后24、48或73小时，开始对多血质Balb/c小鼠的原代脾细胞进行培养。这些细胞在细胞培养约64、48或23小时时，分别表现出明确的血红素合成波。对这些培养物中脾集落形成病毒（SFFV）和辅助性鼠白血病病毒（MuLV-F）产生情况的检测显示，两种病毒的最高产生速率与血红素合成峰值基本同时出现。病毒产生峰值和血红素合成峰值在体外出现的时间与感染后的时间间隔（80 - 105小时）有关，而非与细胞接种到细胞培养中的时间或细胞培养的小时数有关。换液实验表明，在这个体外系统中，血红素合成与病毒产生之间的时间关系是FV感染细胞的一个内在特征。
