Vasilyev Aleksandr, Drummond Iain A
Nephrology Division and Department of Pathology, Charlestown, MA, USA.
Methods Mol Biol. 2012;886:55-70. doi: 10.1007/978-1-61779-851-1_6.
The zebrafish has emerged as a powerful model to study organ development and regeneration. It has a number of advantages over other vertebrate model systems. The embryo can be kept transparent throughout embryonic development, which allows direct visualization of the developing organs. In addition, embryos can be easily manipulated surgically, genetically, or chemically. Furthermore, because nephron shape and function are remarkably conserved among vertebrates, zebrafish findings can directly inform human studies. Here, we describe a simple procedure that can be used by laboratories to investigate the development of zebrafish kidney and other organs by time-lapse microscopy.
斑马鱼已成为研究器官发育和再生的强大模型。与其他脊椎动物模型系统相比，它具有许多优势。在整个胚胎发育过程中，胚胎可保持透明，这使得能够直接观察发育中的器官。此外，胚胎可以很容易地进行手术、基因或化学操作。而且，由于脊椎动物的肾单位形状和功能显著保守，斑马鱼的研究结果可以直接为人类研究提供信息。在这里，我们描述了一种简单的程序，各实验室可利用该程序通过延时显微镜研究斑马鱼肾脏及其他器官的发育。
