研究培养的后肾间充质细胞中的初级纤毛。
Investigating primary cilia in cultured metanephric mesenchymal cells.
作者信息
Chi Lijun, Rosenblum Norman
机构信息
Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
出版信息
Methods Mol Biol. 2012;886:157-63. doi: 10.1007/978-1-61779-851-1_14.
PMID:22639259
Abstract
Primary cilia are present in most vertebrate cells. They have complex structures that are required for signal transduction in developing tissues. The embryonic kidney consists of two major cell lineages, ureteric and metanephric mesenchyme. Here, we describe a method to isolate metanephric mesenchyme from ureteric bud, culture metanephric mesenchyme cells, and study primary cilia in cell culture.
摘要
原发性纤毛存在于大多数脊椎动物细胞中。它们具有复杂的结构，这些结构是发育组织中信号转导所必需的。胚胎肾由两个主要细胞谱系组成，即输尿管芽和后肾间充质。在这里，我们描述了一种从输尿管芽中分离后肾间充质、培养后肾间充质细胞并在细胞培养中研究原发性纤毛的方法。
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