Ortiz-Muñoz Guadalupe, Mallavia Beñat, Lopez-Franco Oscar, Hernandez-Vargas Purificacion, Egido Jesus, Gomez-Guerrero Carmen
Renal and Vascular Research Lab, IIS-Fundacion Jimenez Diaz. Autonoma University, Madrid, Spain.
Methods Mol Biol. 2012;886:321-9. doi: 10.1007/978-1-61779-851-1_29.
Renal gene therapy may offer new strategies to treat diseases of native and transplanted kidneys. Several experimental techniques have been developed using viral, nonviral, and cellular vectors, although the effectiveness of such techniques varies widely depending upon the vector used, type of injection, species, and experimental model of renal disease. Here, we describe an optimized technique for renal delivery of DNA in rodents by retrograde renal vein injection as it is currently applied in our laboratory for adenovirus and nonviral vectors. This is an effective gene transfer method with lasting effect on gene expression in the kidney that modulates renal disease in rodents without any apparent harmful effect, thus having a potential therapeutic value for future clinical applications.
肾脏基因治疗可能为治疗天然肾脏和移植肾脏疾病提供新策略。已经开发了几种使用病毒、非病毒和细胞载体的实验技术，尽管这些技术的有效性因所使用的载体、注射类型、物种和肾脏疾病实验模型的不同而有很大差异。在此，我们描述一种通过逆行肾静脉注射将DNA递送至啮齿动物肾脏的优化技术，目前该技术在我们实验室用于腺病毒和非病毒载体。这是一种有效的基因转移方法，对肾脏中的基因表达具有持久影响，可调节啮齿动物的肾脏疾病且无任何明显有害影响，因此对未来临床应用具有潜在治疗价值。
