Department of Animal Science, Aarhus University, Foulum, P.O. Box 50, DK-8830, Tjele, Denmark.
Biol Trace Elem Res. 2012 Dec;150(1-3):147-53. doi: 10.1007/s12011-012-9453-2. Epub 2012 May 26.
The objectives of this experiment were to study bioavailability of trace elements in beans and wheat containing different levels of zinc and to study how the water solubility of trace elements was related to the bioavailability in pigs. Three wheat and two bean types were used: wheat of Danish origin as a control (CtrlW), two Turkish wheat types low (LZnW) and high (HZnW) in zinc, a common bean (Com), and a faba bean (Faba). Two diets were composed by combining 81 % CtrlW and 19 % Com or Faba beans. Solubility was measured as the trace element concentration in the supernatant of feedstuffs, and diets incubated in distilled water at pH 4 and 38°C for 3 h. The bioavailability of zinc and copper of the three wheat types and the two bean-containing diets were evaluated in the pigs by collection of urine and feces for 7 days. The solubility of zinc was 34-63 %, copper 18-42 %, and iron 3-11 %. The zinc apparent digestibility in pigs was similar in the three wheat groups (11-14 %), but was significantly higher in the CtrlW+Faba group (23 %) and negative in the CtrlW+Com group (-30 %). The apparent digestibility of copper was higher in the HZnW (27 %) and CtrlW+Faba (33 %) groups than in the CtrlW (17 %) and LZnW (18 %) groups. The apparent copper digestibility of the CtrlW+Com diet was negative (-7 %). The solubility and digestibility results did not reflect the concentration in feedstuffs. The in vitro results of water solubility showed no relationship to the results of trace mineral bioavailability in pigs.
本实验的目的是研究不同锌水平的豆类和小麦中微量元素的生物利用度，并研究微量元素的水溶性与猪体内的生物利用度之间的关系。使用了三种小麦和两种豆类：丹麦小麦作为对照（CtrlW）、两种锌含量低（LZnW）和高（HZnW）的土耳其小麦、普通豆（Com）和蚕豆（Faba）。通过将 81%的 CtrlW 和 19%的 Com 或 Faba 豆组合成两种日粮。在 pH 4 和 38°C 下，用蒸馏水在 3 小时内孵育饲料，测量微量元素在饲料上清液中的浓度，以确定其溶解度。通过收集猪的尿液和粪便，在 7 天内评估三种小麦类型和两种含豆日粮中锌和铜的生物利用率。锌的溶解度为 34-63%，铜为 18-42%，铁为 3-11%。猪对三种小麦组中锌的表观消化率相似（11-14%），但在 CtrlW+Faba 组（23%）较高，在 CtrlW+Com 组（-30%）较低。HZnW（27%）和 CtrlW+Faba（33%）组的铜表观消化率高于 CtrlW（17%）和 LZnW（18%）组。CtrlW+Com 日粮的铜表观消化率为负值（-7%）。溶解度和消化率的结果与饲料中的浓度无关。体外水溶性结果与猪体内微量元素生物利用度的结果没有关系。
