Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongdajie Street, Fengtai District, Beijing 100071, PR China.
Neurotoxicol Teratol. 2012 Jul;34(4):442-9. doi: 10.1016/j.ntt.2012.05.053. Epub 2012 May 25.
Chlorpyrifos (CPF) is one of the most commonly used insecticides throughout the world and has become one of the major pesticides detected in farm products. Chronic exposures to CPF, especially at the dosages without eliciting any systemic toxicity, require greater attention. The purpose of this study was, therefore, to evaluate the behavioral effects of repeated low doses (doses that do not produce overt signs of cholinergic toxicity) of CPF in adult rats. Male rats were given 0, 1.0, 5.0 or 10.0mg/kg of CPF through intragastric administration daily for 4 consecutive weeks. The behavioral functions were assessed in a series of behavioral tests, including water maze task, open-field test, grip strength and rotarod test. Furthermore, the present study was designed to evaluate the effects of repeated exposures to CPF on water maze recall and not acquisition. The results showed that the selected doses only had mild inhibition effects on cholinesterase activity, and have no effects on weight gain and daily food consumption. Performances in the spatial retention task (Morris water maze) were impaired after the 4-week exposure to CPF, but the performances of grip strength and rotarod test were not affected. Motor activities in the open field were changed, especially the time spent in the central zone increased. The results indicated that repeated exposures to low doses of CPF may lead to spatial recall impairments, behavioral abnormalities. However, the underlying mechanism needs further investigations.
毒死蜱（CPF）是世界上使用最广泛的杀虫剂之一，已成为农产品中检测到的主要农药之一。慢性暴露于 CPF，特别是在没有引起任何全身毒性的剂量下，需要引起更多关注。因此，本研究旨在评估成年大鼠重复低剂量（不产生明显胆碱能毒性的剂量）CPF 的行为影响。雄性大鼠通过灌胃每天给予 0、1.0、5.0 或 10.0mg/kg 的 CPF，连续 4 周。通过一系列行为测试评估行为功能，包括水迷宫任务、旷场试验、握力和转棒试验。此外，本研究旨在评估重复接触 CPF 对水迷宫回忆而不是获取的影响。结果表明，所选剂量仅对胆碱酯酶活性有轻度抑制作用，对体重增加和每日食物消耗没有影响。在 4 周 CPF 暴露后，空间保留任务（Morris 水迷宫）的表现受损，但握力和转棒试验的表现不受影响。旷场中的运动活动发生改变，特别是中央区域的停留时间增加。结果表明，重复接触低剂量 CPF 可能导致空间回忆受损和行为异常。然而，其潜在机制需要进一步研究。
