Department of Genetics, Graduate University for Advanced Studies, Sokendai, Japan.
Yeast. 2012 Jun;29(6):241-9. doi: 10.1002/yea.2907. Epub 2012 May 29.
Measuring relative genetic distances is one of the best ways to locate genetic loci. Here we report the construction of a strains set for genetic mapping in Schizosaccharomyces japonicus, which belongs to the genus Schizosaccharomyces together with the well-studied fission yeast Sz. pombe. We constructed 29 strains that bear a positive-negative selection marker at different loci. The marker was inserted every 500 kb in the genome of Sz. japonicus. Each marker thus becomes a 'scale mark' of a chromosome that behaves like a yardstick. By determining the genetic distances from the inserted markers, the relative location of a genomic mutation can be determined. We also constructed a fosmid library that covers an entire genome of Sz. japonicus. These tools together would facilitate identification and cloning of the gene.
衡量相对遗传距离是定位遗传基因座的最佳方法之一。在此，我们报告了构建一个用于在裂殖酵母属中的裂殖酵母（Schizosaccharomyces japonicus）进行遗传作图的品系库的情况。裂殖酵母与研究得较多的有丝分裂酵母（Schizosaccharomyces pombe）同属裂殖酵母属。我们构建了 29 个菌株，这些菌株在不同的基因座上带有正-负选择标记。标记每隔 500kb 插入到 Sz. japonicus 的基因组中。因此，每个标记都成为染色体的“比例尺标记”，其行为类似于量尺。通过确定插入标记之间的遗传距离，可以确定基因组突变的相对位置。我们还构建了一个覆盖 Sz. japonicus 整个基因组的 fosmid 文库。这些工具一起将有助于鉴定和克隆基因。
