Khalili Mohsen, Jalali Mohammad Reza, Mirzaei-Azandaryani Mohammad
Department of Physiology, Medicinal Plant, School of Medicine, Shahed University of Medical Sciences, Tehran, Iran.
Urol J. 2012 Spring;9(2):472-9.
To investigate the effects of the hydroalcoholic extract of Hypericum perforatum (H. perforatum) leaves on the kidney calculi in rats.
Fifty-two Wistar male rats were randomly divided into 4 groups: Groups 2, 3, and 4 received ethylene glycol (EG) 1% + ammonium chloride (0.5%)-added drinking water as a stone inducer. The H. Perforatum solution was fed at the same time of EG application and was repeated once for two days until the end of the experimental period. Group 1 (control; n = 10) was provided free access to food and normal drinking water. Group 2 (EG; n = 12) received EG-ammonium chloride-added drinking water and was fed with normal chow. Groups 3 and 4 (n = 15), as EG-treatment groups, received H. Perforatum in low (300 mg/kg) and high (500 mg/kg) doses, respectively. After 28 days, the kidneys were removed and prepared for histologic evaluation of calcium oxalate deposits.
Urine level of free calcium in groups EG and EG + H. perforatum (300 mg/kg) and phosphorous in EG + H. perforatum (500 mg/kg) significantly decreased compared to controls (P < .01; P < .05; and P < .05, respectively). Treatment of the rats with high dose of H. Perforatum (500 mg/kg) markedly reduced decrementing effect of EG on serum level of free calcium (P < .05). Histological experiments showed that chronic feeding of H. perforatum (300 and 500 mg/kg, orally) could significantly reduce the size and number of calcium oxalate deposits in EG group.
Chronic treatment of rats with hydroalcoholic extract of H. perforatum reduced the size and number of calcium oxalate deposits in EG-induced calculi.
研究贯叶连翘叶水醇提取物对大鼠肾结石的影响。
52只雄性Wistar大鼠随机分为4组：第2、3、4组饮用添加1%乙二醇（EG）和0.5%氯化铵的水以诱导结石形成。贯叶连翘溶液在给予EG的同时投喂，每两天重复一次，直至实验期结束。第1组（对照组；n = 10）自由进食和饮用正常水。第2组（EG组；n = 12）饮用添加EG - 氯化铵的水，并给予普通饲料。第3组和第4组（n = 15）作为EG治疗组，分别给予低剂量（300 mg/kg）和高剂量（500 mg/kg）的贯叶连翘。28天后，取出肾脏，准备进行草酸钙沉积的组织学评估。
与对照组相比，EG组、EG + 贯叶连翘（300 mg/kg）组的尿游离钙水平以及EG + 贯叶连翘（500 mg/kg）组的尿磷水平显著降低（分别为P < .01；P < .05；和P < .05）。高剂量贯叶连翘（500 mg/kg）处理大鼠显著降低了EG对血清游离钙水平的降低作用（P < .05）。组织学实验表明，长期口服贯叶连翘（300和500 mg/kg）可显著减少EG组草酸钙沉积物的大小和数量。
用贯叶连翘水醇提取物长期处理大鼠可减少EG诱导结石中草酸钙沉积物的大小和数量。
