Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, 3500-3 Minamitamagaki-cho, Suzuka, Mie 513-8670, Japan.
Anticancer Res. 2012 Jun;32(6):2343-6.
Cholesterol-dependent cytolysins (CDCs) are secreted from various types of bacteria and are involved in various diseases (e.g. abscess formation). Traditional CDCs has a conserved 11mer region, which is a key structure in membrane recognition.
Based on the X-ray data of traditional CDC perfringolysin O (PFO), molecular models of intermedilysin (ILY), pyolysin (PLO), vaginolysin (VLY), and Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) were constructed. The 11mer regions of these models were extracted, and their molecular features were analyzed.
The dipole moments of these 11mer regions were classified into four types, and their stereo-hydrophobicity (dGW) was different. It was thought that these results influenced the species specificity and membrane recognition of each cytolysin.
Traditional CDCs, ILY, PLO, and VLY consisted of four domains (domains 1 to 4). Domain 0 existed on the N-terminal side in Sm-hPAF in addition to these four domains. The 11mer sequence of Sm-hPAF is the same as that of VLY, but Sm-hPAF has slightly different characteristics (e.g. species specificity, membrane recognition, cholesterol dependency) compared to VLY. Dynamic structure analysis of domain 0 might clarify these differences.
胆固醇依赖性细胞毒素（CDCs）由各种类型的细菌分泌，并与各种疾病有关（例如脓肿形成）。传统的 CDC 具有保守的 11mer 区域，这是膜识别的关键结构。
基于传统 CDC 产气荚膜梭菌溶素 O（PFO）的 X 射线数据，构建了白细胞介素（ILY）、细胞溶素（PLO）、阴道溶素（VLY）和链球菌衍生的人血小板聚集因子（Sm-hPAF）的分子模型。提取这些模型的 11mer 区域，并分析其分子特征。
这些 11mer 区域的偶极矩分为四种类型，其立体疏水性（dGW）不同。据认为，这些结果影响了每种细胞毒素的物种特异性和膜识别。
传统的 CDC、ILY、PLO 和 VLY 由四个结构域（结构域 1 到 4）组成。与这些四个结构域相比，Sm-hPAF 除了这四个结构域外，N 端还存在结构域 0。Sm-hPAF 的 11mer 序列与 VLY 相同，但 Sm-hPAF 与 VLY 相比具有略微不同的特征（例如，物种特异性、膜识别、胆固醇依赖性）。结构域 0 的动态结构分析可能会阐明这些差异。
