Department of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Anticancer Res. 2012 Jun;32(6):2385-90.
Immunotherapy using cytotoxic T-lymphocytes (CTLs) still has limited success. An increase in the frequency of CTL administration is one method to improve immunotherapy using CTLs. The conventional method (C-method) that generates CTLs after the induction of dendritic cells requires a long time period. If CTLs can be more rapidly and simply induced, the frequency of immunotherapy could be increased and unexpected contamination could be avoided. In this study, in order to more rapidly induce functional CTLs, we investigated a new method (N-method) that uses a cytokine cocktail, including interleukin (IL)-2, IL-4, granulocyte macrophage colony-stimulating factor, tumour necrosis factor-α and interferon-α, together with a tumour lysate. CTLs induced by the N-method had equivalent functions, such as proliferation, surface antigen expression and cytotoxicity, compared with those induced by the C-method. These results suggest that the N-method can substitute the C-method in order to improve the effect of immunotherapy using CTLs.
细胞毒性 T 淋巴细胞（CTLs）的免疫疗法仍然成效有限。增加 CTL 的给药频率是提高 CTL 免疫疗法的一种方法。生成树突状细胞后诱导 CTLs 的传统方法（C 方法）需要很长时间。如果能够更快、更简单地诱导 CTLs，那么免疫疗法的频率就可以增加，并且可以避免意外污染。在这项研究中，为了更快速地诱导功能性 CTLs，我们研究了一种新方法（N 方法），该方法使用细胞因子鸡尾酒，包括白细胞介素（IL）-2、IL-4、粒细胞巨噬细胞集落刺激因子、肿瘤坏死因子-α和干扰素-α，以及肿瘤裂解物。与 C 方法诱导的 CTLs 相比，N 方法诱导的 CTLs 具有等效的功能，如增殖、表面抗原表达和细胞毒性。这些结果表明，N 方法可以替代 C 方法，以提高 CTLs 免疫疗法的效果。
