Functional Genomics and Cancer Biology Laboratory, Center for Medical Engineering, Vellore Institute of Technology, Vellore, Tamil Nadu, India.
Nutr Cancer. 2012;64(5):714-24. doi: 10.1080/01635581.2012.686648. Epub 2012 May 29.
Curcumin has long been used as an antioxidative, antiinflammatory, and modulator of pathological angiogenesis, whereas naringenin is a well-known immunomodulator. In this report, we investigated the effect of curcumin and naringenin on the growth of Ehrlich ascites carcinoma tumor model. To achieve this, Swiss albino mice were implanted intraperitoneally with 1 × 10⁶ Ehrlich ascites carcinoma cells followed by the administration of oral doses of naringenin and curcumin either individually (50 mg/kg body weight) or in combination (20 mg/kg body weight each). A marked reduction has been seen in the total number of cells (80%) and accumulation of ascetic fluid (55%) when these drugs were administered together. These drugs proved to be an effective angio-inhibitory compound and confirmed by different in vivo assay systems, viz. peritoneal/skin angiogenesis and chorioallantoic membrane assay. Antiangiogenic and antiproliferative effect of these compounds alone or in combination was further corroborated with immunoblot results where we confirmed the downregulation of vascular endothelial growth factor, Hif1α, heat shock protein 90, and p-Akt. Furthermore, treatment with naringenin and curcumin alone or in combination substantially improved hepatocellular architecture and no noticeable neoplastic lesions or cellular alteration were reported. These outcomes put forward a plausible clinical application of these diet-derived compounds, as both angioinhibitory and antitumor in association with conventional therapy.
姜黄素长期以来一直被用作抗氧化剂、抗炎剂和病理性血管生成的调节剂，而柚皮素则是一种众所周知的免疫调节剂。在本报告中，我们研究了姜黄素和柚皮素对艾氏腹水癌肿瘤模型生长的影响。为了实现这一目标，我们将瑞士白化病小鼠腹膜内植入 1×10⁶ 艾氏腹水癌细胞，然后分别给予柚皮素和姜黄素的口服剂量（50mg/kg 体重）或联合剂量（20mg/kg 体重）。当这些药物联合使用时，观察到总细胞数（80%）和腹水积聚（55%）明显减少。这些药物被证明是一种有效的血管生成抑制化合物，并通过不同的体内检测系统，即腹膜/皮肤血管生成和绒毛尿囊膜 assay 得到证实。这些化合物单独或联合使用的抗血管生成和抗增殖作用，通过免疫印迹结果得到进一步证实，其中我们证实了血管内皮生长因子、Hif1α、热休克蛋白 90 和 p-Akt 的下调。此外，柚皮素和姜黄素单独或联合治疗显著改善了肝细胞结构，没有报告明显的肿瘤病变或细胞改变。这些结果提出了这些饮食来源的化合物具有合理的临床应用前景，因为它们与传统治疗联合具有血管抑制和抗肿瘤作用。
