Department of Biological Sciences, Australia.
J Ethnopharmacol. 2012 Jul 13;142(2):539-47. doi: 10.1016/j.jep.2012.05.034. Epub 2012 May 27.
The extract from the stem bark of Garcinia buchananii trees is used as an anti-diarrhea remedy in sub-Saharan Africa. We tested the hypothesis that G. buchananii bark extract and its anti-motility fractions are effective treatments against lactose-induced diarrhea.
A high-lactose (35%) diet was used to induce diarrhea in Wistar rats, which were then treated with either G. buchananii bark extract (0.1, 0.5, 1.0 and 5.0 g bark powder), and its anti-motility fractions isolated using preparative thin layer chromatography; termed PTLC1 (15 mg) and PTLC5 (3.8 mg) or loperamide (8.4 mg). Drug preparations were dissolved in 1L except PTCL1 and PTLC5 that were dissolved in 100mL tap water. Numerous parameters were measured in each condition including consistency, fluid and mucus content of feces, body weight, water and food consumption, urine production and bloating.
Diarrheic rats produced watery or loose, mucuoid, sticky, feces. Fluids constituted 86% of stool mass compared with only 42% for control rats fed standard chow. Compared with controls, diarrheic rats produced more urine, lost weight and had bloated ceca and colons. All doses of the extract, its anti-motility fractions and loperamide individually stopped diarrhea within 6-24 h of administration, whilst significantly reducing mucus and fecal fluid content, urine production and intestinal bloating. Rats treated with 0.1g extract, PTLC1 and PTLC5 gained weight, whilst PTLC5 also increased water intake.
Garcinia buchananii extract and its anti-motility fractions are effective remedies against lactose-induced diarrhea. The extract contains compounds that reverse weight loss, promote food and water intake, supporting the notion that characterization of the compounds could lead to new therapies against diarrheal diseases.
来源于藤黄属树木茎干的提取物被用于治疗撒哈拉以南非洲的腹泻。我们假设藤黄属树木茎干提取物及其抗运动成分是治疗乳糖诱导性腹泻的有效方法，并对这一假设进行了测试。
使用高乳糖（35%）饮食诱导 Wistar 大鼠腹泻，然后用藤黄属树木茎干提取物（0.1、0.5、1.0 和 5.0g 树皮粉末）和使用制备性薄层色谱法分离的其抗运动成分进行治疗；分别命名为 PTLC1（15mg）和 PTLC5（3.8mg）或洛哌丁胺（8.4mg）。药物制剂除 PTCL1 和 PTLC5 溶解在 100mL 自来水中外，均溶解在 1L 中。在每种情况下测量了许多参数，包括粪便的稠度、液体和黏液含量、体重、水和食物消耗、尿量和腹胀。
腹泻大鼠产生水样或松散、黏液样、粘稠、粪便。与食用标准饲料的对照大鼠相比，粪便中液体占 86%，而仅占 42%。与对照组相比，腹泻大鼠产生更多的尿液、体重减轻、盲肠和结肠肿胀。提取物、其抗运动成分和洛哌丁胺的所有剂量在给药后 6-24 小时内均能停止腹泻，同时显著减少黏液和粪便液体含量、尿量和肠道肿胀。用 0.1g 提取物、PTLC1 和 PTLC5 治疗的大鼠体重增加，而 PTLC5 还增加了水的摄入量。
藤黄属树木提取物及其抗运动成分是治疗乳糖诱导性腹泻的有效方法。提取物含有可逆转体重减轻、促进食物和水摄入的化合物，这表明对这些化合物的特性进行描述可能会导致针对腹泻疾病的新疗法。
