Ozawa Yoshiharu, Arima Hiroshi, Banno Ryoichi, Ito Yoshihiro, Goto Motomitsu, Morishita Yoshiaki, Sugimura Yoshihisa, Ozaki Nobuaki, Nagasaki Hiroshi, Oiso Yutaka
Department of Endocrinology and Diabetes, Nagoya University School of Medicine, Showa-ku, Nagoya, Japan.
Neuroreport. 2012 Aug 1;23(11):673-5. doi: 10.1097/WNR.0b013e3283556676.
The area postrema (AP) is a circumventricular organ that lacks a blood-brain barrier. Previous studies have shown that the lesion of AP (APX) attenuated hyperphagic responses to glucoprivation. As the orexigenic neuropeptide Y (NPY) neurons have been implicated in the regulation of food intake, we examined whether the activation of NPY neurons by glucoprivation is mediated through the AP as well. In agreement with previous studies, hyperphagic responses to an injection of 2-deoxy-D-glucose that blocks glucose utilization were significantly attenuated in the APX group compared with the sham-operated (Sham) group. However, the expression levels of NPY heteronuclear RNA, a sensitive indicator for the gene transcription, were significantly increased in the arcuate nucleus by a 2-deoxy-D-glucose injection in both the APX and the Sham groups, and there were no significant differences in the values between groups. These data suggest that the hyperphagic response to glucoprivation, but not the activation of NPY gene transcription in the arcuate nucleus, was mediated through the AP in the hindbrain.
最后区（AP）是一个缺乏血脑屏障的室周器官。先前的研究表明，最后区损伤（APX）减弱了对糖剥夺的摄食亢进反应。由于促食欲神经肽Y（NPY）神经元与食物摄入的调节有关，我们也研究了糖剥夺对NPY神经元的激活是否也通过最后区介导。与先前的研究一致，与假手术（Sham）组相比，APX组对注射2-脱氧-D-葡萄糖（其可阻断葡萄糖利用）的摄食亢进反应显著减弱。然而，在APX组和Sham组中，注射2-脱氧-D-葡萄糖后，作为基因转录敏感指标的NPY异核RNA的表达水平在弓状核中均显著增加，且两组间的值无显著差异。这些数据表明，对糖剥夺的摄食亢进反应是通过后脑的最后区介导的，但弓状核中NPY基因转录的激活并非如此。
