Watanabe Minemori, Arima Hiroshi, Ozawa Yoshiharu, Goto Motomitsu, Shimizu Hiroshi, Banno Ryoichi, Sugimura Yoshihisa, Ozaki Nobuaki, Nagasaki Hiroshi, Oiso Yutaka
Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
Neurosci Lett. 2009 Oct 16;464(1):6-9. doi: 10.1016/j.neulet.2009.08.005. Epub 2009 Aug 5.
While the hypothalamus has been implicated in the regulation of energy balance, the central mechanisms and neural circuit that coordinate the feeding response to energy deficit have not been fully clarified. To better understand the role of the hypothalamus in mediating hyperphagic responses to food deprivation or glucoprivation, we examined the feeding responses in rats in which the medial hypothalamus (MH) was isolated from the rest of the brain. The isolation of the MH was performed with a Halasz's knife cut, and experiments were performed 7 days after the operation. Food consumption between 9:00 a.m. and 11:00 a.m. in rats which had been fasted overnight was significantly increased compared to that in rats which had access to food ad libitum before the measurement in both the sham and MH-isolated groups, and the absolute values of food consumption in fasted rats were not significantly different between the groups. On the other hand, while an injection of 2-deoxy-d-glucose, which blocks glucose utilization, significantly increased food consumption for 2h after injection compared to a saline injection in the sham group, it did not increase food intake compared to saline injection in the MH-isolated groups. Thus, it is demonstrated that glucoprivation is not an effective stimulus to induce feeding in MH-isolated rats.
虽然下丘脑与能量平衡的调节有关，但协调对能量不足的进食反应的中枢机制和神经回路尚未完全阐明。为了更好地理解下丘脑在介导对食物剥夺或糖剥夺的贪食反应中的作用，我们检测了内侧下丘脑（MH）与大脑其他部分分离的大鼠的进食反应。采用哈拉斯氏刀切法分离MH，并在手术后7天进行实验。在假手术组和MH分离组中，与测量前自由进食的大鼠相比，隔夜禁食的大鼠在上午9:00至11:00的食物消耗量均显著增加，且禁食大鼠的食物消耗绝对值在两组之间无显著差异。另一方面，虽然注射阻断葡萄糖利用的2-脱氧-D-葡萄糖后，与假手术组注射生理盐水相比，在注射后2小时内显著增加了食物消耗量，但与MH分离组注射生理盐水相比，并未增加食物摄入量。因此，证明糖剥夺不是诱导MH分离大鼠进食的有效刺激。
