Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Anal Bioanal Chem. 2012 Sep;404(4):991-1009. doi: 10.1007/s00216-012-6070-z. Epub 2012 May 27.
Mass spectrometry has proven to be an indispensable tool for protein identification, characterization, and quantification. Among the possible methods in quantitative proteomics, stable isotope labeling by using reductive dimethylation has emerged as a cost-effective, simple, but powerful method able to compete at any level with the present alternatives. In this review, we briefly introduce experimental and software methods for proteome analysis using dimethyl labeling and provide a comprehensive overview of reported applications in the analysis of (1) differential protein expression, (2) posttranslational modifications, and (3) protein interactions.
质谱已被证明是蛋白质鉴定、特性分析和定量的不可或缺的工具。在定量蛋白质组学中可能的方法中，利用还原二甲基化进行稳定同位素标记已经成为一种具有成本效益、简单但强大的方法，能够在任何层面上与当前的替代方法竞争。在这篇综述中，我们简要介绍了使用二甲基标记进行蛋白质组分析的实验和软件方法，并全面概述了报告的在分析（1）差异蛋白表达、（2）翻译后修饰和（3）蛋白相互作用中的应用。
