Department of Thoracic Surgery, Provincial Hospital Affiliated with Shandong University, Shandong, China.
Ann Surg Oncol. 2012 Oct;19(11):3606-12. doi: 10.1245/s10434-012-2419-y. Epub 2012 May 30.
Esophageal squamous cell carcinoma (ESCC) is a malignant tumor with a high incidence of lymph node metastasis. This study was undertaken to investigate the expression of CCR7 and VEGF-C in pN0 ESCC and its relationship with lymphatic metastatic recurrence.
The expression of CCR7 and VEGF-C was examined by RT-PCR and immunohistochemistry. The recurrence rates were calculated by the Kaplan-Meier method and their difference was determined by log rank analysis. Cox regression analysis was performed to determine the independent risk factors.
In 99 patients, CCR7 mRNA expression was observed in 42 patients with a 3 year recurrence rate of 57.1%; VEGF-C mRNA expression was observed in 52 patients with a 3 year recurrence rate of 53.8%; and coexpression of CCR7 mRNA and VEGF-C mRNA was observed in 22 patients with a 3 year recurrence of 63.6%. Neither CCR7 mRNA nor VEGF-C mRNA expression was observed in 27 patients with a 3 year recurrence rate of 22.2%. The recurrence rates of patients with positive expression of CCR7 mRNA and/or VEGF-C mRNA were significantly higher than in patients without expression of both CCR7 mRNA and VEGF-C mRNA. We achieved better concordance between RT-PCR and immunohistochemistry detection of both markers. The Cox regression analysis showed tumor T classification, positive expression of CCR7/VEGF-C mRNA, and positive expression of CCR7/VEGF-C protein in tumor tissues to be independent risk factors for 3 year recurrence.
Patients with positive expression of CCR7 and/or VEGF-C have a higher recurrence rate than patients without expression of both CCR7 and VEGF-C. CCR7 and VEGF-C may become molecular indicators of disease in patients vulnerable to lymphatic metastatic recurrence.
食管鳞状细胞癌（ESCC）是一种具有高淋巴结转移发生率的恶性肿瘤。本研究旨在探讨 CCR7 和 VEGF-C 在 pN0 ESCC 中的表达及其与淋巴转移复发的关系。
采用 RT-PCR 和免疫组织化学方法检测 CCR7 和 VEGF-C 的表达。采用 Kaplan-Meier 法计算复发率，对数秩分析比较差异，Cox 回归分析确定独立危险因素。
在 99 例患者中，42 例患者 CCR7mRNA 表达阳性，3 年复发率为 57.1%；52 例患者 VEGF-CmRNA 表达阳性，3 年复发率为 53.8%；22 例患者同时表达 CCR7mRNA 和 VEGF-CmRNA，3 年复发率为 63.6%。27 例患者未检测到 CCR7mRNA 和 VEGF-CmRNA 的表达，3 年复发率为 22.2%。CCR7mRNA 和/或 VEGF-CmRNA 阳性表达患者的复发率明显高于 CCR7mRNA 和 VEGF-CmRNA 均阴性表达的患者。我们在 RT-PCR 和免疫组织化学检测这两种标志物方面取得了更好的一致性。Cox 回归分析显示，肿瘤 T 分期、CCR7/VEGF-CmRNA 阳性表达和肿瘤组织中 CCR7/VEGF-C 蛋白阳性表达是 3 年复发的独立危险因素。
CCR7 和/或 VEGF-C 阳性表达的患者复发率高于 CCR7 和 VEGF-C 均阴性表达的患者。CCR7 和 VEGF-C 可能成为易发生淋巴转移复发患者疾病的分子指标。
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