活细胞中天冬氨酸激酶 A 的生物正交成像。
Bioorthogonal imaging of aurora kinase A in live cells.
机构信息
Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA.
出版信息
Angew Chem Int Ed Engl. 2012 Jul 2;51(27):6598-603. doi: 10.1002/anie.201200994. Epub 2012 May 29.
Abstract
: Aurora kinase A (AKA) was imaged in live cells using an bioorthogonal two-step reaction with a small molecule and a fluorescent reporter. The small molecule was localized to spindle poles and microtubules during metaphase, consistent with the localization of both endogenous and GFP-/RFP-tagged AKA. Using this approach, changes in AKA distribution during mitosis were also observed.
摘要
使用小分子和荧光报告分子的生物正交两步反应在活细胞中对极光激酶 A（AKA）进行成像。在中期，小分子定位于纺锤体极和微管，与内源性和 GFP-/RFP 标记的 AKA 的定位一致。通过这种方法，还观察到 AKA 在有丝分裂过程中的分布变化。
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