Kuz'mina V V, Shalygin M V, Skvortsova E G
Zh Evol Biokhim Fiziol. 2012 Mar-Apr;48(2):129-34.
Effect of temperature on proteinases activities of enteral microbiota and of intestinal mucosa was studied in five fish species (roach Rutilus rutilus, crucian carp Carassius carassius, common perch Perca fluviatilis, pike-perch Zander lucioperca, and pike Esox lucius) belonging by the nutrition type to different ecological groups. Essential differences of temperature characteristics of proteinases of intestinal mucosa and of enteral microbiota are revealed in fish belonging by the nutrition type to different ecologic groups. The character of the t0-function of proteinases of intestinal mucosa and enteral microbiota by casein and hemoglobin as a rule is different. The highest values of relative proteinases activities for casein in the zone of low temperatures (38 and 45.3 % of the maximal activity) are found at study of proteinases of enteral microbiota in common perch and crucian carp. The latter indicates a significant adaptability of the enteral microbiota proteinases of common perch and crucial carp to functioning at low temperatures.
研究了温度对五种营养类型属于不同生态组的鱼类（拟鲤Rutilus rutilus、鲫鱼Carassius carassius、河鲈Perca fluviatilis、梭鲈Zander lucioperca和白斑狗鱼Esox lucius）肠道微生物群和肠黏膜蛋白酶活性的影响。在营养类型属于不同生态组的鱼类中，揭示了肠黏膜和肠道微生物群蛋白酶温度特征的本质差异。通常情况下，肠黏膜和肠道微生物群蛋白酶以酪蛋白和血红蛋白为底物时的t0函数特征不同。在研究河鲈和鲫鱼肠道微生物群蛋白酶时，发现低温区（最大活性的38%和45.3%）酪蛋白相对蛋白酶活性的最高值。后者表明河鲈和鲫鱼肠道微生物群蛋白酶对低温环境具有显著的适应性。
