Rajabi Mehdi, Gorincioi Elena, Santaniello Enzo
Dipartimento di Scienze Chimiche, Alimentari, Farmaceutiche e Farmacologiche, Universitá del Piemonte Orientale, Novara, Italy.
Nucleosides Nucleotides Nucleic Acids. 2012;31(6):474-81. doi: 10.1080/15257770.2012.681825.
Cytokinins and cytokinin nucleosides are purine derivatives with potential anticancer activity both in vitro and in vivo. N(6)-furfuryladenosine (kinetin riboside, KR) displays antiproliferative and apoptogenic activity against various human cancer cell lines and has recently been shown to suppress tumor growth in murine xenograft models of human leukemia and melanoma. In this study, we demonstrate that KR is able to inhibit the proliferation in HCT-15 human colon cancer cells in a dose-dependent manner with a concentration of 2.5 μM, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that KR arrested cell cycle progression in the S Phase by blocking through G(2)/M and G(0)/G(1) phase in HCT-15 colon cells. Moreover, suppression of clonogenic activity occurs after exposure to KR at a concentration of 2.5 μM for HCT-15.
细胞分裂素和细胞分裂素核苷是嘌呤衍生物，在体外和体内均具有潜在的抗癌活性。N(6)-糠基腺苷（激动素核糖苷，KR）对多种人类癌细胞系具有抗增殖和诱导凋亡活性，最近已显示其可抑制人白血病和黑色素瘤小鼠异种移植模型中的肿瘤生长。在本研究中，我们证明KR能够以剂量依赖的方式抑制HCT-15人结肠癌细胞的增殖，浓度为2.5μM时可导致50%的细胞活力抑制。通过流式细胞术进行的细胞周期分析表明，KR通过阻断HCT-15结肠细胞的G(2)/M和G(0)/G(1)期，使细胞周期进程停滞在S期。此外，HCT-15细胞在暴露于2.5μM的KR后，克隆形成活性受到抑制。
